Anti-ATP12A antibody produced in rabbit

The ATPase H⁺/K⁺ transporting non-gastricα2 subunit (ATP12A) gene, with 23 exons and 22 introns spanning 32kb of genomic DNA, is mapped to human chromosome 13q12.1-q12.3. ATP12A is a member of P-type cation transport ATPases family. The gene encodes the catalytic alpha subunit of Na⁺/K⁺-ATPase, which is ubiquitously expressed.

ATPase, H+/K+ transporting, nongastric, alpha polypeptide recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper) and immunofluorescence.

ATPase H⁺/K⁺ transporting non-gastric α2 subunit (ATP12A) plays a vital role in the regulation of acid-base and K⁺ homeostasis in the kidney and colon. In addition, it also aids acidification of prostate secretions in the anterior lobe of the organ and transepithelial bicarbonate secretion in the ducts of the exocrine pancreas. ATP12A mutations might affect myocardial Na⁺ handling and subsequently myocardial function. Overexpression of ATP12A has been observed in human colorectal carcinoma.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST81283.

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

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