Anti-LRRC45 antibody produced in rabbit

The gene LRRC45 (leucine rich repeat containing 45) encodes a 72kDa protein that contains several N-terminal leucine-rich repeat (LRR) domains and a long coiled-coil domain at the C terminus. It is localized at the proximal ends of the mother and daughter centrioles forming fiber-like structures. It also forms a ring-like structure at the distal end of the mother centriole. The gene is mapped to human chromosome 17q25.3.

Leucine-rich repeat-containing protein 45 recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

The gene LRRC45 (leucine rich repeat containing 45) encodes a centrosome linker that facilitates centrosome cohesion. It associates with the proteins C-Nap1 (centrosomal Nek2-associated protein 1) and rootletin, which are phosphorylated and dissociated from the centrosome by Nek2A (NIMA related kinase 2A) at the beginning of mitosis. This results in the displacement of LRRC45 from the centrosome during mitosis. Lack of LRRC45 protein leads to centrosome splitting during interphase.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST75796

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

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