Anti-TBX20 antibody produced in rabbit

T-box transcription factor (TBX20) belongs to the T-box transcription factor family. It is expressed in the developing hearts of embryos in vertebrates and invertebrates. TBX20 contains a DNA-binding domain called the T-box domain and the gene encoding this protein is present on chromosome 7p14-p15.

T-box transcription factor TBX20 recombinant protein epitope signature tag (PrEST)

Anti-TBX20 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

T-box transcription factor (TBX20) activates cardiac gene expression by interacting with transcription factors NK2 homeobox 5 (Nkx2-5), GATA binding protein 4 (GATA4) and GATA binding protein 5 (GATA5). During endocardial differentiation, it specifically interacts with GATA5. TBX20 and other T-box genes and factors interact repressively. This is responsible for splitting of the myocardium into the chamber and non-chamber. It is an important event upon which all the subsequent morphogenesis activities depend. Based on the particular isoform expressed and cellular requirements, TBX20 can regulate transcription of target genes both positively and negatively. Its T-box DNA-binding domain associates specifically with the consensus DNA half site sequence defined for the brachyury (T) protein. TBX20 also interacts with homeodomain factor Nkx2-5 and zinc finger factor GATA4.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Corresponding Antigen APREST70963.

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.