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Merck
CN

H9950

Sigma-Aldrich

Hanahan′s Broth (SOB Medium)

Liquid microbial growth medium

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UNSPSC代码:
12352200

等级

for molecular biology

无菌性

sterile; 0.2 μm filtered

形式

liquid

适用性

suitable for nonselective for Escherichia coli and Coliforms

储存温度

room temp

一般描述

Hanahan′s (SOB) media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

应用

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

特点和优势

Hanahan′s (SOB) media provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

组分

20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4

外形

20 g/L Tryptone
5.0 g/L Yeast Extract
10mM NaCl
2.5mM KCl
10mM MgCl2
10mM MgSO4

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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