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生物来源
human
质量水平
重组
expressed in NSO cells
检测方案
≥95% (SDS-PAGE)
形式
lyophilized powder
效能
20-40 ng/mL
质量
endotoxin tested
分子量
70-80 kDa by SDS-PAGE (non-reducing)
包装
pkg of 5 and 25 μg
储存条件
avoid repeated freeze/thaw cycles
技术
cell culture | mammalian: suitable
杂质
<1.0 EU/μg
UniProt登记号
储存温度
−20°C
基因信息
human ... HGF(3082)
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一般描述
Recombinant, human hepatocyte growth factor (HGF) is produced from the 728 amino acid residue variant of the human pre-pro-HGF protein 6 and expressed in a mouse myeloma cell line NSO.The HGF gene is mapped to human chromosome 7q21.1. The encoded protein is a mesenchymal-fibroblast derived cytokine.
应用
Hepatocyte Growth Factor human has been used as a growth factor cocktail to suspend rat fibrogen/thrombin cells. It has also been used to study the tyrosine phosphorylation of MET (MET proto-oncogene, receptor tyrosine kinase) in myxofibrosarcoma cell line.
生化/生理作用
HGF′s major function is to induce in vitro hepatocytes proliferation. HGF is also essential for cell migration and angiogenesis. Itis associated with embryonic development and tissue homeostasis. It is known to mediate placental trophoblast invasion, which is essential for normal fetal growth. Abnormal and defective HGF causes preeclampsia due to placental insufficiency. HGF exhibits its multifunctions through c-MET (proto-oncogene tyrosine kinase receptor). HGF is known to induce human umbilical vein endothelial cell proliferation and vasculogenic mimicry migration in gastric cancer cells, thus serving as a good target for cancer therapy. Upregulation of the HGF gene is observed in advanced stages of cancer, such as pancreatic adenocarcinoma.
肝细胞生长因子（HGF）是肝细胞和多种细胞类型（包括内皮和上皮细胞、黑素细胞和角质形成细胞）的有效促分裂原。它还可介导上皮形态发生。HGF是由met原癌基因编码的酪氨酸激酶受体的配体。
其他说明
NSO表达的重组HGF含有大于95％的异二聚体HGF。昆虫细胞系表达的重组HGF是单链HGF（主要）和一些异二聚体HGF的混合物。单链HGF和异二聚体HGF在 体外 分析中具有相同的活性。
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine.
分析说明
The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
HGF derived from cancer-associated fibroblasts promotes vascularization in gastric cancer via PI3K/AKT and ERK1/2 signaling
Ding X, et al.
Oncology Reports (2018)
Prognostic implication of MET overexpression in myxofibrosarcomas: an integrative array comparative genomic hybridization, real-time quantitative PCR, immunoblotting, and immunohistochemical analysis
Lee JC, et al.
Modern Pathology, 23(10), 1379-1379 (2010)
Analysis of hepatocyte growth factor immunostaining in the placenta of HIV-infected normotensive versus preeclamptic pregnant women
Cele SB,et al.
Eur. J. Obstet. Gyn. Rep. Biol. (2018)
Correlation between HGF/c-Met and Notch1 signaling pathways in human gastric cancer cells
Huang KH, et al.
Oncology Reports, 40(1), 294-302 (2018)
Dual inhibition of Hedgehog and c-Met pathways for pancreatic cancer treatment
Rucki AA, et al.
Molecular Cancer Therapeutics (2017)
商品
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.
Role of growth factors in stem cell differentiation and various growth factors for your research at sigmaaldrich.com
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