产品名称
单克隆抗-HA 小鼠抗, clone HA-7, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
HA-7, monoclonal
contains
15 mM sodium azide
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
- 人胚肾 293 细胞的免疫沉淀
- HeLa 细胞免疫印迹
- 人胚肾 293 细胞的免疫纯化
- 小鼠胚胎成纤维细胞电子显微镜免疫染色
- 海马切片的免疫细胞化学
- 免疫印迹，研究融合蛋白的表达
- 免疫共沉淀试验
- 裂解物蛋白的免疫印迹
Biochem/physiol Actions
Disclaimer
General description
Immunogen
Preparation Note
若长期保存，分装冷冻保存。不建议反复冻融或存放在“无霜”冰柜中。如果长期储存时出现轻微浑浊，请在使用前通过离心澄清溶液。工作稀释液如果在12个小时内未使用，则应丢弃。
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
What is the recommended dilution of Product H9658 - HA antibody for immunoprecipitation and for indirect immunostaining?
H9658 - Monoclonal Anti-HA antibody produced in mouse is the ascites fluid. It is not purified. We only test this product in western blotting. This product contains 15-55 mg/ml total protein with 4-7 mg/ml specific antibody. H3663 - Monoclonal Anti-HA antibody produced in mouse as the purified immunoglobulin. We test this product for immunoprecipitation using 0.5-2.0 μg and for immunocytochemistry using 1.0-2.0 μg/mL.These ranges would be a good starting concentration. In order to obtain best results in different techniques and preparations, we recommend determining optimal working dilution by titration test.
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Instructions
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