推荐产品
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
HAP46.5, monoclonal
形式
buffered aqueous solution
分子量
antigen ~25 kDa
种属反应性
canine, mouse, rat, chicken, human
技术
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extract of human MCF7 breast carcinoma cells
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... HMGB1(3146)
mouse ... Hmgb1(15289)
rat ... Hmgb1(25459)
一般描述
High-mobility group-1 protein (HMG-1) or amphoterin is a nuclear DNA-binding protein that facilitates the bending and transcription of DNA. It also has a pivotal role in the regulation of chromatin structure, DNA replication, ligation and repair. Monoclonal anti-HMG-1 antibody can be used as a vital tool to study the biology of transcription. It can also be used in indirect ELISA and immunoblotting (25kD approx.). Mouse anti-HMG-1 antibody reacts specifically with HMG-1 of canine, mouse, chicken, rat and human.
Monoclonal Anti-HMG-1 (mouse IgG1 isotype) is derived from the HAP46.5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with purified human recombinant HMG-1. HMG-1 (High-Mobility-Group protein, amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMG-1. It is a prevalent non-histone chromatin component and a non-sequence specific DNA binding protein. HMG-1 consists of two homologous HMG boxes rich in basic amino acids and an acidic tail at the carboxy-terminus.
免疫原
purified recombinant human HMG-1.
应用
Monoclonal Anti-HMG-1 antibody is suitable for use in immunochemical applications such as ELISA and immunoblotting (approx. 25 kDa).
Monoclonal anti-HMG-1 antibody can be used in immunofluorescent staining. It can also be used in microarray and western blotting.
生化/生理作用
High-Mobility-Group protein, amphoterin, HMG-1 is involved in the regulation of chromatin structure as well as being involved, either as positive or negative factors with various aspects of DNA replication, transcription, repair, and ligation. HMG-1, identified as a membrane associated protein termed “amphoterin,” mediates neurite outgrowth, tumor outgrowth, and metastasis. It participates in plasminogen activation and is recognized as a late mediator of endotoxin lethality in mice.
适用性
Monoclonal Anti-HMG-1 recognizes human, canine, mouse, rat, and chicken HMG-1.
外形
Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Immunology letters, 121(1), 61-73 (2008-09-30)
Although anti-DNA antibodies have been decisively linked to the pathogenesis of lupus nephritis, the mechanisms have not been conclusively determined. Recently, we reported that anti-DNA antibodies may contribute to kidney damage by upregulation of proinflammatory genes in mesangial cells (MC)
Non-histone nuclear factor HMGB1 is phosphorylated and secreted in colon cancers
Laboratory Investigation; a Journal of Technical Methods and Pathology, 89(8), 948-948 (2009)
Molecular medicine reports, 13(6), 5335-5341 (2016-04-29)
High mobility group box 1 (HMGB1) protein serves an important role in myocardial ischemia/reperfusion (I/R) injury. MicroRNAs (miRNAs) are a group of small non‑coding RNAs that regulate numerous signaling pathways involved in myocardial I/R injury. The present study aimed to investigate whether
Tissue antigens, 70(2), 151-156 (2007-07-06)
High-mobility group box 1 protein (HMGB1) is a nuclear DNA-binding protein, which also functions as a pleiotropic cytokine, implicated in the pathology of several different immune-mediated diseases. The purpose of this study was to examine the HMGB1 gene for putative
Oncology letters, 12(3), 2133-2138 (2016-09-08)
microRNA (miR/miRNA) are small non-coding RNAs that control gene expression at the post-transcriptional level by targeting mRNAs. Aberrant expression of miRNAs is often observed in different types of cancer. Specific miRNAs function as tumor suppressors or oncogenes and interfere with
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