推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~140 kDa
种属反应性
human, mouse, rat
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
技术
immunoprecipitation (IP): 10-20 μg using RIPA extract of HeLa nuclei
indirect immunofluorescence: 1:250 using HEK 293T cells expressing recombinant mouse HDAC4
microarray: suitable
western blot: 1:1,000 using whole extracts of mouse NIH3T3 cells
western blot: 1:500 using whole extracts of rat brain
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... HDAC4(9759)
mouse ... Hdac4(208727)
rat ... Hdac4(363287)
一般描述
Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position.
Mammalian histone deacetylases (HDACs) are classified into three classes according to sequence homology. HDAC1, HDAC2, HDAC3 and HDAC8 belong to class I HDACs and HDAC4, HDAC6, and HDAC7 belong to class II HDACs. Class III consists of the yeast sirtuins (sir2-like protein). Class I HDACs are ubiquitously expressed. Whereas, most of the class II HDACs show tissue-specific expression. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
特异性
Anti-Histone Deacetylase 4 (HDAC4), recognizes human, mouse and rat HDAC4.
免疫原
Synthetic peptide corresponding to amino acid residues of human HDAC4 with C-terminal added lysine conjugated to KLH.
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below:
- Western Blotting
- Immnofluorescence
- Immunoprecipitation
生化/生理作用
Histone deacetylase 4 (HDAC4) contains import and export nuclear signal that aids in nucleocytoplasmic shuttling. It has physical and functional interactions with transcription factor myocyte enhancer factor-2 (Mef2) protein and 14-3-3. This interaction disturbs nucleocytoplasmic shuttling and then guides HDAC4 to the cytoplasm and the nucleus, respectively. In addition, HDAC4 with HDAC5 and HDAC7 represses transcription by binding to the nuclear receptor co-repressors silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) and nuclear receptor co-repressor (NCOR).
Studies show that HDAC4 contains a composed import and export nuclear signal that aids in nucleocytoplasmic shuttling. It has physical and functional interactions with transcription factor MEF2C and 14-3-3. This interaction results in the shuttling and it guides HDAC4 to the cytoplasm and the nucleus.The C-terminal domain of HDAC4 shows innate histone deacetylase activity. It is seen that MEF2C combines with HDAC4 at the N-terminal domain and downmodulate c-jun promoter activity.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
储存及稳定性
For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
Michal Mielcarek et al.
PloS one, 6(11), e27746-e27746 (2011-12-06)
Huntington's disease (HD) is a progressive neurological disorder for which there are no disease-modifying treatments. Transcriptional dysregulation is a major molecular feature of HD, which significantly contributes to disease progression. Therefore, the development of histone deacetylase (HDAC) inhibitors as therapeutics
Hdac4 interactions in Huntington's Disease viewed through the prism of multiomics
<BIG>Federspiel JD, et al.</BIG>
Molecular and Cellular Proteomics (2019)
Class II and IV HDACs function as inhibitors of osteoclast differentiation
Blixt NC, et al.
PLoS ONE, 12(9), e0185441-e0185441 (2017)
Carina Mello Guimaraes Meyers et al.
Bone, 159, 116393-116393 (2022-03-24)
Protein kinase D (PRKD) family kinases are required for formation and function of osteoclasts. However, the substrates of PRKD in osteoclasts are unknown. To identify PRKD-dependent protein phosphorylation in osteoclasts, we performed a quantitative LC-MS/MS phosphoproteomics screen for proteins showing
A H Wang et al.
Molecular and cellular biology, 21(17), 5992-6005 (2001-08-04)
Nucleocytoplasmic trafficking of histone deacetylase 4 (HDAC4) plays an important role in regulating its function, and binding of 14-3-3 proteins is necessary for its cytoplasmic retention. Here, we report the identification of nuclear import and export sequences of HDAC4. While
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