生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~140 kDa (additional weaker bands may be present)
种属反应性
rat, human, mouse
增强验证
independent
Learn more about Antibody Enhanced Validation
技术
immunoprecipitation (IP): 10-20 μg using RIPA extract of HeLa nuclei
indirect immunofluorescence: 1:250 using HeLa cells
microarray: suitable
western blot: 1:1,000 using whole extracts of mouse NIH3T3 cells
western blot: 1:500 using whole extracts of rat brain
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... HDAC4(9759)
mouse ... Hdac4(208727)
rat ... Hdac4(363287)
相关类别
一般描述
Mammalian histone deacetylases (HDACs) can be divided into three classes according to sequence homology. Class II consists of the yeast Hda1-like proteins histone deacetylase 4,5,6,7,8,9, 10 (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9, and HDAC10.
免疫原
synthetic peptide corresponding to amino acid residues 1-19 of human HDAC4 with C-terminal added lysine conjugated to KLH.
应用
Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
- immunostaining
- immunohistochemistry
- chromatin immunoprecipitation
生化/生理作用
Histone deacetylase 4 (HDAC4) exhibits intrinsic nuclear import and export signals for its dynamic nucleocytoplasmic shuttling. Its association with 14-3-3 and monocyte enhancer factor 2 (MEF2) proteins can disturb shuttling and thus directs HDAC4 to the cytoplasm and the nucleus, respectively.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
常规特殊物品
Lilian Varricchio et al.
The international journal of biochemistry & cell biology, 50, 112-122 (2014-03-07)
To clarify the role of HDACs in erythropoiesis, expression, activity and function of class I (HDAC1, HDAC2, HDAC3) and class IIa (HDAC4, HDAC5) HDACs during in vitro maturation of human erythroblasts were compared. During erythroid maturation, expression of HDAC1, HDAC2
Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoR
Fischle W, et al.
Molecular Cell, 9(1), 45-57 (2002)
Stress-induced epigenetic transcriptional memory of acetylcholinesterase by HDAC4
Sailaja BS, et al.
Proceedings of the National Academy of Sciences of the USA, 109(52), E3687-E3695 (2012)
Histone deacetylase inhibitors preserve white matter structure and function during ischemia by conserving ATP and reducing excitotoxicity
Baltan S, et al.
The Journal of Neuroscience, 31(11), 3990-3999 (2011)
HDAC4 governs a transcriptional program essential for synaptic plasticity and memory
Sando III R, et al.
Cell, 151(4), 821-834 (2012)
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