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Merck
CN

H9037

Sigma-Aldrich

抗-HA−TRITC 抗体,小鼠单克隆 小鼠抗

~1 mg/mL, clone HA-7, purified from hybridoma cell culture

别名:

单克隆抗-HA, 抗-HA, 抗-流感血凝素

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.56

生物来源

mouse

质量水平

偶联物

TRITC conjugate

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

HA-7, monoclonal

表单

buffered aqueous solution

储存条件

protect from light

浓度

~1 mg/mL

技术

direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

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一般描述

单克隆抗-HA来源于小鼠骨髓瘤细胞和BALB/c小鼠脾细胞融合产生的HA-7杂交瘤。单克隆抗HA通过免疫细胞化学识别HA标记的融合蛋白上的HA标签序列。该抗体与N-端和C-端HA标签融合蛋白特异反应。

特异性

该抗体可识别HA-标签蛋白的天然和还原变性形式,并且可与 大肠杆菌 或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。

免疫原

与人流感病毒血凝素(HA)片段相对应的合成肽,称为HA-标签,与KLH结合

应用

单克隆抗HA-TRITC抗体用于测试DOT/ICM效应子SDHA是否对于大蜡螟和A/J小鼠中嗜肺军团菌的毒力是必需的。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
免疫荧光分析(1篇论文)

外形

溶于含有1%牛血清白蛋白和15mM叠氮化钠(作为防腐剂)的0.01M磷酸盐缓冲生理盐水(pH 7.4)中的溶液。

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储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
含少量动物源组分生物产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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D J Scott et al.
British journal of urology, 58(2), 119-124 (1986-04-01)
To assess the effectiveness of the surgical correction of vesico-ureteric reflux, current renal function was determined in 56 children with scarred kidneys who had undergone ureteric reimplantation between 1978 and 1983. The children were aged between 2 and 15 years
Clare R Harding et al.
Infection and immunity, 81(7), 2598-2605 (2013-05-08)
Legionella pneumophila is an intracellular bacterium that resides within amoebae and macrophages in a specialized compartment termed the Legionella-containing vacuole (LCV). As well as providing an intracellular niche for replication, the LCV helps to prevent the release of bacterial components
Yasuhiro Yamazaki et al.
Science immunology, 5(44) (2020-03-01)
We investigated the molecular and cellular basis of severe combined immunodeficiency (SCID) in six patients with otofaciocervical syndrome type 2 who failed to attain T cell reconstitution after allogeneic hematopoietic stem cell transplantation, despite successful engraftment in three of them.
P O Olins et al.
Current opinion in biotechnology, 4(5), 520-525 (1993-10-01)
Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by altering heterologous gene-coding sequences. Fusion technology
Félix Matadamas-Martínez et al.
Memorias do Instituto Oswaldo Cruz, 115, e190348-e190348 (2020-02-13)
It was previously demonstrated that CMC-20, a nitazoxanide and N-methyl-1H-benzimidazole hybrid molecule, had higher in vitro activity against Giardia intestinalis WB strain than metronidazole and albendazole and similar to nitazoxanide. To evaluate the in vitro activity of CMC-20 against G.

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