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一般描述
卤代烃油700用于在琼脂糖垫上安装蠕虫。它便于蠕虫在显微镜下移动。在光学数字胚胎取样过程中,卤代烃充当氧源并阻止干燥。
应用
卤代烃油700已用于:
- 促进用玻璃毛细管刺穿卵黄膜,以在蜘蛛胚胎上制造小孔
- 在显微注射程序中跟踪秀丽隐杆线虫中的疱疹性口炎病毒感染
- 用于使用蔡司LSM710共焦系统对胚胎样本进行活体成像
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Drosophila melanogaster: Practical Uses in Cell and Molecular Biology, 514-514 (1995)
C. elegans: A Practical Approach, 81-81 (1999)
Frontiers in neuroscience, 12, 324-324 (2018-06-06)
Here we described an experimental protocol for in vivo imaging of macropinocytosis and subsequent intracellular events. By microinjection, we delivered fluorescence dextrans together with or without ATPγS into transparent Drosophila melanogaster embryos. Using a confocal microscope for live imaging, we
eLife, 9 (2020-11-27)
Class I ventral posterior dendritic arborisation (c1vpda) proprioceptive sensory neurons respond to contractions in the Drosophila larval body wall during crawling. Their dendritic branches run along the direction of contraction, possibly a functional requirement to maximise membrane curvature during crawling
Lab on a chip, 17(13), 2303-2322 (2017-06-15)
We present a microfluidic chip for immobilizing Drosophila melanogaster larvae for high resolution in vivo imaging. The chip creates a low-temperature micro-environment that anaesthetizes and immobilizes the larva in under 3 minutes. We characterized the temperature distribution within the chip
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