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Merck
CN

H8651

Sigma-Aldrich

HEPES钠盐 钠盐

BioXtra, ≥99.0% (titration)

别名:

4-(2-羟乙基)哌嗪-1-乙磺酸 钠盐, N-(2-羟乙基)哌嗪-N′-(2-乙磺酸) 钠盐

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About This Item

经验公式(希尔记法):
C8H17N2NaO4S
CAS号:
分子量:
260.29
EC 号:
MDL编号:
UNSPSC代码:
12161700
PubChem化学物质编号:
NACRES:
NA.25

产品线

BioXtra

方案

≥99.0% (titration)

表单

powder

杂质

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

pH值(酸碱度)

10.0-12.0 (1 M in H2O)

有效pH范围

6.8-8.2

pKa (25 °C)

7.5

溶解性

H2O: 1 M, clear, colorless

痕量阴离子

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

痕量阳离子

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

吸附

≤0.2 at 260 in H2O at 1 M
≤0.2 at 280 in H2O at 1 M

应用

diagnostic assay manufacturing

SMILES字符串

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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一般描述

HEPES 是一种通用两性离子缓冲液,不结合镁、钙、锰 (II) 或铜 (II) 离子。HEPES 已被Norman Good 博士和他的同事在 1966 年分类为 Good 缓冲液。在生物 pH 值为两性离子,在 pH 6.8~8.2 范围内最有效。

应用

HEPES 钠盐溶液适用于细胞培养研究。可用于制备 HEPES 缓冲液。可在透化成纤维细胞的磷酸化试验中用作缓冲液。可组成复合透析缓冲液,用于放射免疫法测定血清中游离甲状腺素。

其他说明

使用 HEPES 钠盐规格表可以非常方便地比较 HEPES 钠盐产品的规格。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor
I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
Jemimah Walker et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 100(4), 1134-1141 (2012-02-15)
Magnesium (Mg) and its alloys have been proposed as degradable replacements to commonly used orthopedic biomaterials such as titanium alloys and stainless steel. However, the corrosion of Mg in a physiological environment remains a difficult characteristic to accurately assess with

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