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Merck
CN

H8651

HEPES钠盐 钠盐

BioXtra, ≥99.0% (titration)

别名:

4-(2-羟乙基)哌嗪-1-乙磺酸 钠盐, N-(2-羟乙基)哌嗪-N′-(2-乙磺酸) 钠盐

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关于此项目

经验公式（希尔记法）:
C8H17N2NaO4S
化学文摘社编号:
75277-39-3
分子量:
260.29
NACRES:
NA.25
PubChem Substance ID:
24895527
UNSPSC Code:
12161700
EC Number:
278-169-7
MDL number:
MFCD00036463

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产品名称

HEPES钠盐 钠盐, BioXtra, ≥99.0% (titration)

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

product line

BioXtra

assay

≥99.0% (titration)

form

powder

impurities

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

pH

10.0-12.0 (1 M in H2O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H2O: 1 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

≤0.2 at 260 in H2O at 1 M
≤0.2 at 280 in H2O at 1 M

application(s)

diagnostic assay manufacturing

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Application

HEPES 钠盐溶液适用于细胞培养研究。可用于制备 HEPES 缓冲液。可在透化成纤维细胞的磷酸化试验中用作缓冲液。可组成复合透析缓冲液，用于放射免疫法测定血清中游离甲状腺素。

General description

HEPES 是一种通用两性离子缓冲液，不结合镁、钙、锰 (II) 或铜 (II) 离子。HEPES 已被Norman Good 博士和他的同事在 1966 年分类为 Good 缓冲液。在生物 pH 值为两性离子，在 pH 6.8～8.2 范围内最有效。

Other Notes

使用 HEPES 钠盐规格表可以非常方便地比较 HEPES 钠盐产品的规格。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLBN1373V
SLBQ9422V
SLBK6788V
SLBP1463V
SLBM2559V

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C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor
I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
Wen-Yih Jeng et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 7), 829-838 (2012-07-04)
NkBgl, a β-glucosidase from Neotermes koshunensis, is a β-retaining glycosyl hydrolase family 1 enzyme that cleaves β-glucosidic linkages in disaccharide or glucose-substituted molecules. β-Glucosidases have been widely used in several applications. For example, mutagenesis of the attacking nucleophile in β-glucosidase
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