一般描述
N-羟基琥珀酰-琼脂糖™ 4 Fast Flow是一种交联预活化的基质，通过间隔臂将Sepharose 4 Fast Flow与6-氨基己酸混合获得。与N-羟基琥珀酰的酯化反应激活了末端羧基。含有配体的基直接与该活性酯相互作用，形成化学性质非常稳定的酰胺键。
应用
N-羟基琥珀酰-琼脂糖™ 4 Fast Flow已被用作基于磁颗粒的视觉检测系统的一部分，以实现扩增基因结合后的分离。它也被用来固定蛋白质在拉下试验。
特点和优势
- 特别推荐用于工艺规模应用中含氨基的小蛋白和多肽的偶联
- 高水平的预激活，这提供了所选配体的高度替代
- NHS偶联方法使配体的化学稳定附着
法律信息
Sepharose is a trademark of Cytiva
警示用语：
Danger
危险声明
危险分类
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
3 - Flammable liquids
WGK
WGK 1
闪点(°F)
53.6 °F
闪点(°C)
12 °C
个人防护装备
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
危险化学品
Seulggie Choi et al.
Molecular immunology, 48(15-16), 2207-2213 (2011-07-12)
Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified
Woo-Hyeon Byeon et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(2), 361-363 (2004-05-12)
Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to
Yoo Min Park et al.
Biosensors & bioelectronics, 141, 111415-111415 (2019-06-16)
We focused on the development of a hand-held pathogen-detection device using smartphone-embedded electronic elements combined with functionalized magnetic particles (MPs) and sepharose. To perform affinity chromatography for evaluating DNA amplicons, avidin-conjugated MPs and succinimide-linked sepharose were used with biotin-primers. To
Yoo Min Park et al.
Talanta, 195, 97-102 (2019-01-11)
The current study focuses on developing a system for visually detecting an amplified bacterial (Escherichia coli O157:H7) gene using a heavy metal particle (MP) and functionalized porous sepharose gel. To functionalize DNA-specificity to the MP, an avidin-modified MP was employed
Julia V Hartig et al.
Nucleic acids research, 39(9), 3836-3851 (2011-01-20)
In Drosophila, siRNAs are classified as endo- or exo-siRNAs based on their origin. Both are processed from double-stranded RNA precursors by Dcr-2 and then loaded into the Argonaute protein Ago2. While exo-siRNAs serve to defend the cell against viruses, endo-siRNAs
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