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安全信息

H7411

Sigma-Aldrich

单克隆抗-HA

clone HA-7, purified from hybridoma cell culture

别名:

抗-HA, 抗-流感血凝素

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About This Item

MDL编号:
MFCD00803873
UNSPSC代码:
12352203
NACRES:
NA.56

生物来源

mouse

质量水平

200

偶联物

FITC conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

HA-7, monoclonal

形式

buffered aqueous solution

储存条件

protect from light

浓度

1 mg/mL

技术

immunocytochemistry: 1-5 μg/mL using HA tagged fusion protein transfected into mammalian cells fixed with paraformaldehyde.

运输

wet ice

储存温度

−20°C

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一般描述

Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry.
Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase.

特异性

该抗体可识别HA-标签蛋白的天然和还原变性形式，并且可与 大肠杆菌 或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。

免疫原

与人流感病毒血凝素(HA)片段相对应的合成肽，称为HA-标签，与KLH结合

应用

Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used:
  • in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells
  • in western blotting detection of spindle-associated proteins in Trypanosoma brucei
  • in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies

生化/生理作用

Hemagglutinin (HA) induces immune response post viral infection. Fluorescein isothiocyanate (FITC) conjugated with the influenza virus HA is majorly used in intracellular tracking of the virus and also in flow cytometric studies.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

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WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品
常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?

    Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Faithful chromosome segregation in Trypanosoma brucei requires a cohort of divergent spindle-associated proteins with distinct functions
Zhou Q, et al.
Nucleic Acids Research, 46(16), 8216-8231 (2018)
Use of FITC-labeled influenza virus and flow cytometry to assess binding and internalization of virus by monocytes-macrophages and lymphocytes
Nichols JE, et al.
Archives of Virology, 130(3-4), 441-455 (1993)
Annalena La Porte et al.
Journal of virology, 90(21), 9889-9904 (2016-08-26)
INI1/hSNF5/SMARCB1/BAF47 is an HIV-specific integrase (IN)-binding protein that influences HIV-1 transcription and particle production. INI1 binds to SAP18 (Sin3a-associated protein, 18 kDa), and both INI1 and SAP18 are incorporated into HIV-1 virions. To determine the significance of INI1 and the
Aortic aneurysm generation in mice with targeted deletion of integrin-linked kinase in vascular smooth muscle cells
Shen D, et al.
Circulation Research, 109(6), 616-628 (2011)
Irina Kufareva et al.
Methods in enzymology, 570, 389-420 (2016-02-29)
Despite the recent breakthrough advances in GPCR crystallography, structure determination of protein-protein complexes involving chemokine receptors and their endogenous chemokine ligands remains challenging. Here, we describe disulfide trapping, a methodology for generating irreversible covalent binary protein complexes from unbound protein
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