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质量水平
方案
≥99.0% (titration)
表单
powder
储存条件
dry at room temperature
技术
HPLC: suitable
颜色
white
pH值（酸碱度）
10-12 (1 M in water)
有效pH范围
6.8-8.2
pKa (25 °C)
7.5
溶解性
water: 0.1 M, clear, colorless
适用性
suitable for mass spectrometry
应用
diagnostic assay manufacturing
general analytical (
)
life science and biopharma
metabolomics
储存温度
room temp
SMILES字符串
[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
InChI key
RDZTWEVXRGYCFV-UHFFFAOYSA-M
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一般描述
HEPES盐，或称4-(2-羟乙基)哌嗪-1-乙烷磺酸钠盐，是一种两性离子生物缓冲液，广泛应用于生物学和生物化学研究。6.8-8.2 pH范围内的缓冲能力使其成为各种研究应用的理想选择。HEPES也是缓冲细胞培养基的常用试剂，其优点在于细胞毒性低于Tris 和磷酸盐等替代品。
HEPES被广泛应用于细胞生物学和生物化学研究，其多功能性在蛋白质纯化和提取过程中得到了充分体现，这一点是由于其在宽pH范围内的稳定性和最小的金属离子结合能力。 此外，HEPES 也是各种生物学和生物化学过程中的重要试剂，包括免疫沉淀、细胞裂解和活细胞成像，这使其成为各种研究应用中的多功能工具。
HEPES被广泛应用于细胞生物学和生物化学研究，其多功能性在蛋白质纯化和提取过程中得到了充分体现，这一点是由于其在宽pH范围内的稳定性和最小的金属离子结合能力。 此外，HEPES 也是各种生物学和生物化学过程中的重要试剂，包括免疫沉淀、细胞裂解和活细胞成像，这使其成为各种研究应用中的多功能工具。
应用
HEPES缓冲液的应用包括：
它已被用于:
- 涉及金属离子的大多数研究
- 涉及金属离子的研究中Tris 和磷酸盐的替代品
- 植物研究中的研磨缓冲液
- 凝胶电泳中的电泳缓冲液
- 电穿孔研究中的缓冲液
- Bradford或BCA 分析
- 用于防止红细胞中蛋白质受损的提取缓冲液
它已被用于:
- 低蔗糖缓冲液制备中的缓冲液成分
- 改良Hanks′溶液（一种假性生理溶液）的缓冲液成分
特点和优势
- 经测试证实重金属污染浓度低，从而确保其适用于各种应用
- 易溶于水
- 金属离子结合能力极低
- 细胞毒性低于其它缓冲液如Tris和磷酸盐
- 在宽pH范围内保持稳定
外形
冻干、喷雾干燥或结晶的材料。
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Essowè Mouzou et al.
Materials science & engineering. C, Materials for biological applications, 61, 564-573 (2016-02-04)
High manganese austenitic steels such as Fe-20Mn-1.2C alloys are among the most promising candidates for biodegradable stents applications due to their high strength, high ductility and their chemical composition. In the current work, 14 day static in-vitro tests were performed
Nishant Mehta et al.
Cell reports, 28(10), 2509-2516 (2019-09-05)
V-domain immunoglobulin (Ig) suppressor of T cell activation (VISTA) is an immune checkpoint protein that inhibits the T cell response against cancer. Similar to PD-1 and CTLA-4, a blockade of VISTA promotes tumor clearance by the immune system. Here, we report a
Camila Jordão Candido et al.
Nutrients, 12(1) (2019-12-22)
Consumption of omega-3 (n-3) polyunsaturated fatty acids (PUFA) is related to improvement in the inflammatory response associated with decreases in metabolic disorders of obesity, such as low-grade inflammation and hepatic steatosis. Linseed (Linum usitatissimum) oil is a primary source of
Ekaterina Eremenko et al.
STAR protocols, 2(3), 100643-100643 (2021-07-27)
The organization of chromatin structure plays a crucial role in gene expression, DNA replication, and repair. Chromatin alterations influence gene expression, and modifications could be associated with genomic instability in the cells during aging or diseases. Here, we provide a
Heejeong Kim et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Selective autolysosomal degradation of damaged mitochondria, also called mitophagy, is an indispensable process for maintaining integrity and homeostasis of mitochondria. One well-established mechanism mediating selective removal of mitochondria under relatively mild mitochondria-depolarizing stress is PINK1-Parkin-mediated or ubiquitin-dependent mitophagy. However, additional
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