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生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
技术
immunoprecipitation (IP): 2.5-4 μg/test using HA-tagged fusion protein from cell lysates
indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells
western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts
应用
research pathology
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
流感血凝素蛋白是一种源自人流感病毒主要刺突膜糖蛋白的九肽。该菌株特异性的糖蛋白是一种84 kDa单体的同源三聚体。每种单体含有两个二硫键连接的亚基:HA1和HA2。
抗-HA是在兔中使用一种对应于偶联至KLH的人流感血凝素(HA)的氨基酸残基的一段合成肽而产生的。采用固定的免疫肽上亲和纯化抗体。
抗-HA抗体对于HA标签融合蛋白的N-端或C-端具有特异性。产品在特定的哺乳动物细胞中可能会检测到交叉反应的条带。该免疫HA肽(货号I12149)可特异性地抑制HA标记蛋白条带的染色。
特异性
免疫原
应用
它还适用于间接免疫荧光(10-20μg/mL,使用HA标签融合蛋白转染的细胞)、免疫沉淀((2.5-4μg/次检测,使用来自细胞裂解物的HA标签融合蛋白)以及蛋白质印迹(0.5-0.8μg/mL,使用HA标签融合蛋白转染的细胞提取物)。
生化/生理作用
HA基因通常用作重组表达载体系统中靶蛋白的一种亲和标签。由这种系统所产生的HA标记蛋白质可产生稳定的不干扰靶蛋白质的功能和分布的融合产物。抗-HA抗体在特定的哺乳动物细胞中可能会检测到交叉反应的条带。该免疫HA肽(货号I12149)可特异性地抑制HA标记蛋白条带的染色。编码HA肽的核酸序列已被插入到各种表达质粒中并与克隆位点相邻,因此能够对HA标记的融合蛋白进行克隆和表达。这样的融合蛋白可在多种生物的细胞中表达:细菌、酵母、昆虫和哺乳动物。在融合蛋白中,HA序列可作为特异性抗体的识别靶点,从而能够实现对HA标记蛋白及相关结合蛋白进行检测、亚细胞定位、表征、定量、功能分析和亲和纯化。在细胞蛋白的不同区域中插入HA表位并随后对完整和通透化处理的细胞中表位的免疫反应性进行检查,可用于研究标记蛋白的细胞表达水平、拓扑结构和功能活性。抗体介导的HA标签检测消除了对新鉴定的、低丰度、不稳定、难以纯化、具有交叉反应性或免疫原性差的蛋白进行耗时的特异性抗体生成的需要。
外形
储存及稳定性
如果更长时间储存,按工作体积分装后冻存。不建议反复冻融。如果在更长时间的储存后出现了轻微的浑浊,在使用前可通过离心而使溶液澄清。稀释的工作溶液在12小时后应当被丢弃。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
从最新的版本中选择一种:
分析证书(COA)
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the concentration of Product No. H6908, anti HA?
The antibody concentration for Product No. H6908 is reported as a lot-specific value on the certificates of analysis. Please refer to the Certificate of Analysis for the lot received for the specific antibody concentration. Values for recent lots have ranged from 0.5 - 0.7 mg/mL.
Is the immunizing peptide for Product H6908, Anti-HA antibody, available?
The immunizing peptide is Product No. I2149. It is useful for displacement of HA-tagged fusion proteins from HA antibody in immunoassays. The successful inhibition of antibody binding by HA peptide demonstrates the binding of the antibody is specific.
What strain of influenza hemagglutinin does Product H6908, Anti-HA antibody, recognize?
The sequence of the immunizing peptide is YPYDVPDYA. This sequence corresponds to amino acids 99-107 of human influenza virus X47 (classified as an H3N2 strain) referred to in Niman, H.L., et al., Proc. Natl. Acad. Sci. USA, 80, 4949-4953, 1983. However, according to the NCBI databases, the same epitope (YPYDVPDYA) corresponds to amino acids 98-106 in the vast majority of prevalent influenza viruses hence our claim in the data sheet of the antibodies. NCBI is National Center for Biotechnology Information.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
相关内容
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蛋白质是细胞内部的主要功能分子,负责细胞功能和生存的多种生物学活动。值得关注的是,许多蛋白质也能与DNA发生相互作用。
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