生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen 15 kDa
种属反应性
mouse, human
技术
microarray: suitable
western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pSer139)
基因信息
human ... H2AFX(3014)
mouse ... H2afx(15270)
一般描述
H2AX占人类细胞中总H2A补体的2-25％，而在发芽酵母中，它实际上构成了所有H2A分子。它具有N末端和C末端区域，以及中心球状结构域。C末端区域有修饰位点，包括乙酰化、生物素化和磷酸化位点。
免疫原
与人组蛋白 H2AX (pSer 139 ) 相应的 C 端（氨基酸 134-142）相对应的合成磷酸化肽。
合成肽序列与 KLH 偶联。免疫原序列在小鼠组蛋白 H2AX 中高度保守（单个氨基酸替换）。
应用
兔生产的抗磷酸组蛋白H2AX（pSer139）抗体可用于免疫荧光和免疫印迹。
生化/生理作用
H2AX对于促进特定DNA修复复合物在受损DNA上的组装至关重要。共济失调毛细血管扩张突变（ATM）是参与DNA双链断裂导致的H2AX磷酸化的主要激酶。H2AX基因敲除小鼠对辐射敏感，并且生长迟缓。它们也具有免疫缺陷，突变的雄性不育。
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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