H5791
肝细胞生长因子 人
HumanKine®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
别名:
HGF, 散射因子, 肝素A
产品名称
肝细胞生长因子 人, HGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤20 ng/mL EC50
quality
endotoxin tested
mol wt
monomer 70 kDa (glycosylated)
packaging
pkg of 5x10 μg
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... HGF(3082)
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Biochem/physiol Actions
肝细胞生长因子（HGF）是肝细胞和多种细胞类型（包括内皮和上皮细胞、黑素细胞和角质形成细胞）的有效促分裂原。它还可介导上皮形态发生。HGF是由met原癌基因编码的酪氨酸激酶受体的配体。
HGF is a multifunctional cytokine that promotes mitogenesis, migration, invasion, and morphogenesis. It is a potent mitogen, stimulating the growth of hepatocytes, renal tubular epithelial cells, epidermal keratinocytes, epidermal melanocytes, Mv1Lu (mink lung epithelial cells), and BALB/MK (mouse keratinocytes). HGF inhibits the growth of B6/F1 (mouse melanoma) cells, KB (human squamous carcinoma) cells, and HepG2 (human hepatoma) cells. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.
Other Notes
NSO表达的重组HGF含有大于95％的异二聚体HGF。昆虫细胞系表达的重组HGF是单链HGF（主要）和一些异二聚体HGF的混合物。单链HGF和异二聚体HGF在 体外 分析中具有相同的活性。
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5.
General description
The HGF gene spans ∼70 kb and consists of 18 exons interrupted by 17 introns. The organization of the human HGF gene is highly homologous to that of human plasminogen. HGF maps to the long arm of human chromosome 7, 7q21.1.
Physical form
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Preparation Note
HumanKine HGF is expressed as a glycosylated 70 kDa single chain monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Karène Mahtouk et al.
Biochimica et biophysica acta, 1806(2), 208-219 (2010-07-27)
Hepatocyte growth factor (HGF) and its receptor MET are essential during embryonic development and throughout postnatal life. However, aberrant MET activation, due to overexpression, mutations, or autocrine ligand production, contributes to the development and progression of a variety of human
T Nakamura et al.
Nature, 342(6248), 440-443 (1989-11-23)
Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. The partial purification
Frieda Kage et al.
Journal of cell science, 135(15) (2022-08-17)
Cell migration frequently involves the formation of lamellipodia induced by Rac GTPases activating WAVE regulatory complex (WRC) to drive Arp2/3 complex-dependent actin assembly. Previous genome editing studies in B16-F1 melanoma cells solidified the view of an essential, linear pathway employing
MET and PI3K/mTOR as a potential combinatorial therapeutic target in malignant pleural mesothelioma.
Rajani Kanteti et al.
PloS one, 9(9), e105919-e105919 (2014-09-16)
Malignant pleural mesothelioma (MPM) is an aggressive disease with a poor prognosis. Studies have shown that both MET and its key downstream intracellular signaling partners, PI3K and mTOR, are overexpressed in MPM. Here we determined the combinatorial therapeutic efficacy of
Hiroshi Kusunoki et al.
Hypertension research : official journal of the Japanese Society of Hypertension, 37(6), 500-506 (2014-03-14)
The paracrine loop involving inflammatory cytokines between adipocytes and macrophages establishes a vicious cycle that aggravates pro-inflammatory changes in adipose tissue. The serum level of hepatocyte growth factor (HGF) is increased in metabolic syndrome, but whether HGF is beneficial or
实验方案
Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.
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