biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
0.02-0.08 ng/mL ED50
quality
endotoxin tested
mol wt
dimer 21-25 kDa (glycosylated)
packaging
pkg of 5 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CSF3(1440)
Biochem/physiol Actions
粒细胞集落刺激因子（G-CSF）是受限嗜中性粒细胞系的造血细胞的增殖、分化、存活和激活因子。它是由被内毒素（LPS）激活的巨噬细胞、被TNF-α和IFNγ激活的单核细胞、被IL-1或TNF-α945;激活的成纤维细胞和内皮细胞以及被IL-1或LPS激活的骨髓基质细胞产生的。这表示，在炎症过程中，内毒素刺激组织巨噬细胞产生G-CSF，同时还产生多种其他细胞因子，包括IL-1和TNF-α，这些细胞因子进一步刺激内皮细胞和成纤维细胞释放更多的G-CSF。G-CSF还可以与IL-3发挥协同作用，缩短早期造血祖细胞的G0期。除了同名的增殖活性外，G-CSF还作用于成熟的中性粒细胞，以提高其存活率并刺激其肿瘤杀伤活性。人类G-CSF结合并激活130 kD至150 kD的糖蛋白单链受体（已被归类为造血（细胞因子）受体家族）、细胞因子I类或gp130相关细胞因子受体家族（尽管它显然不与gp130结合）。G-CSF受体存在于嗜中性粒细胞、响应G-CSF的髓样白血病细胞、嗜中性粒细胞系的骨髓细胞以及胎盘滋养层中，并且可以表达为可溶形式。通过可变剪接能够从单个基因合成两种形式的人G-CSF（177和174个氨基酸），但是鼠G-CSF只能表达为178个氨基酸这一种形式。人和鼠G-CSF具有73%的氨基酸序列同源性和完全交叉反应性。
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (mouse myeloid leukemia indicator cell line).
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Mechanisms of G-CSF-mediated hematopoietic stem and progenitor mobilization.
Greenbaum AM, Link DC.
Leukemia (2010)
Antiopi Varelias et al.
Blood, 125(15), 2435-2444 (2015-02-13)
Idiopathic pneumonia syndrome (IPS) is a relatively common, frequently fatal clinical entity, characterized by noninfectious acute lung inflammation following allogeneic stem cell transplantation (SCT), the mechanisms of which are unclear. In this study, we demonstrate that immune suppression with cyclosporin
Renée Beekman et al.
Blood, 115(25), 5131-5136 (2010-03-20)
Granulocyte colony-stimulating factor (G-CSF) has been used in the clinic for more than 2 decades to treat congenital and acquired neutropenias and to reduce febrile neutropenia before or during courses of intensive cytoreductive therapy. In addition, healthy stem cell donors
Jessy Arbez et al.
Cytotherapy, 17(7), 948-955 (2015-03-31)
This study aimed to characterize the immune effectors contained in the grafts from donor mice mobilized by granulocyte colony-stimulating factor (G-CSF) and plerixafor and to evaluate their impact on the development of acute graft-versus-host-disease (aGVHD). Mobilization was done with G-CSF
Akiko Sugiyama et al.
Chronobiology international, 32(7), 934-941 (2015-07-15)
Circulating hematopoietic stem cells exhibit robust circadian fluctuations, which influence the mobilized cell yield, even during enforced stem cell mobilization. However, alterations in the expression of circadian clock genes during granulocyte colony-stimulating factor (G-CSF)-induced peripheral blood stem cell (PBSC) mobilization
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