Application
组蛋白适用于：
- 作为体外激酶测定的阳性对照，检测Cdc2-细胞周期蛋白B1磷酸化GST-CD-4蛋白的能力
- Cdk5活性测定
- 组蛋白H1激酶测定
- 作为聚ADP-核糖(PAR)受体，检测体外PAR聚合酶活性
- 作为[γ-32P]ATP来源32P的受体，检测小、大黄体细胞来源裂解液的蛋白激酶活性
Biochem/physiol Actions
组蛋白是一组DNA结合蛋白，其特征是赖氨酸和精氨酸含量较高。组蛋白的分子量在11-21 kDa之间，取决于具体级分。分离和表征了五种不同的组蛋白级分。H1富含赖氨酸、H2a和H2b略微富含赖氨酸、H3和H4富含精氨酸。H1用于连接染色质链上的各个“念珠”(nu bodies)。
Features and Benefits
不要将Sigma“类型”名称与Luck及其同事使用的“分级”名称混淆。
Other Notes
经SDS-PAGE鉴定，主要为f1亚型
Preparation Note
富含赖氨酸的级分，分离和描述依据文献（de Nooij, E. and Westenbrink, H.G., BiochimBiophys.Acta, 62, 608 (1962)）。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
Liang Zheng et al.
Haematologica, 105(4), 1107-1119 (2019-11-23)
Thrombotic thrombocytopenic purpura (TTP) is caused by severe deficiency of ADAMTS13 (A13), a plasma metalloprotease that cleaves endothelium-derived von Willebrand factor (VWF). However, severe A13 deficiency alone is often not sufficient to cause an acute TTP; additional factors may be
Q P Dou et al.
Cancer research, 53(7), 1493-1497 (1993-04-01)
Progression of cells into S phase is proposed to be determined by accumulation of a labile protein (the restriction point protein R; A. B. Pardee, Proc. Natl. Acad. Sci. USA, 71: 1286-1290, 1974). We report here that cyclin E and
Anne Cecília Nascimento da Cruz et al.
Pharmaceuticals (Basel, Switzerland), 12(4) (2019-11-21)
Fourteen arylsemicarbazone derivatives were synthesized and evaluated in order to find agents with potential anticancer activity. Cytotoxic screening was performed against K562, HL-60, MOLT-4, HEp-2, NCI-H292, HT-29 and MCF-7 tumor cell lines. Compounds 3c and 4a were active against the
Scott T
Concise encyclopedia of biochemistry, 265-265 (1988)
Charlotte Juillet et al.
Journal of medicinal chemistry, 64(2), 1197-1219 (2021-01-09)
Significant inhibition of Aurora B was achieved by the synthesis of simplified fragments of benzosceptrins and oroidin belonging to the marine pyrrole-2-aminoimidazoles metabolites isolated from sponges. Evaluation of kinase inhibition enabled the discovery of a synthetically accessible rigid acetylenic structural
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