一般描述
洗脱缓冲液是亲和层析中的一种主要溶剂。洗脱缓冲液先用于洗脱未结合的蛋白质，然后更高浓度的洗脱缓冲液用于从配体中释放所需的蛋白质。重点是，洗脱缓冲液起作用速度极快，不会改变所需蛋白质的功能或活性。
应用
HIS-Select® 洗脱缓冲液已被用于重组凝固酶蛋白 和 tomoregulin 的洗脱。
外形
含有 250mM 咪唑，0.3M 氯化钠，50mM 磷酸钠，pH8.0。
法律信息
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Danger
危险声明
危险分类
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Affinity Chromatography
Scouten, William H
Chromatography (1981)
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