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生物来源
bacterial (Flavobacterium heparinum)
质量水平
偶联物
conjugate (Glucosaminoglycan)
表单
lyophilized powder
比活
≥200 units/mg protein
浓度
≥200 unit/mg protein (enzyme + BSA)
运输
dry ice
储存温度
−20°C
一般描述
肝素酶是一种可诱导的、非细胞外的肝素降解酶。三种肝素化是 肝素黄杆菌 产生的 ，含有特定的肝素序列。
应用
来自肝素黄杆菌的肝素酶I和II混合物已被用于：
- 消化绵羊玻璃体的硫酸乙酰肝素
- 人胚胎肾细胞
- 来自动脉组织的糖胺聚糖
- P0 视网膜消化
生化/生理作用
肝素酶 I 和 III 在多种生物学过程中发挥重要作用：调节细胞生长因子相互作用、细胞-脂蛋白相互作用、新生血管形成。 在 2-O-硫酸化 α 存在下裂解高硫酸化多糖链-L-吡喃二糖糖醛酸和 β-多糖的 D-吡喃葡萄糖醛酸残基。
肝素降解裂解酶，识别硫酸肝素蛋白聚糖为其主要底物。
包装
按肝素酶 I 单位销售
单位定义
在 25℃、PH 7.5 下，以肝素、钠作为肝素酶 I 的底物，每小时 1 个单位将形成 0.1 μmol 不饱和糖醛酸。
在 25℃、PH 7.5 下，采用牛肾肝素，硫酸盐作为肝素酶 III 的底物，每小时 1 个单位将形成 0.1 μmol 不饱和糖醛酸。
25°C、pH 7.5 下 1 个单位不饱和糖醛酸形成（0.1 μmol/小时）。1 个国际单位 (I.U.) 相当于约 600σ 单位。包装尺寸以 σ 单位销售。
在 25℃、PH 7.5 下，采用牛肾肝素，硫酸盐作为肝素酶 III 的底物，每小时 1 个单位将形成 0.1 μmol 不饱和糖醛酸。
25°C、pH 7.5 下 1 个单位不饱和糖醛酸形成（0.1 μmol/小时）。1 个国际单位 (I.U.) 相当于约 600σ 单位。包装尺寸以 σ 单位销售。
其他说明
Hep I 和 4.2.2.8 III 型肝炎
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
含少量动物源组分生物产品
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Farizeh Aalam et al.
PLoS pathogens, 16(10), e1008968-e1008968 (2020-10-20)
Despite 25 years of research, the basic virology of Kaposi Sarcoma Herpesviruses (KSHV) in B lymphocytes remains poorly understood. This study seeks to fill critical gaps in our understanding by characterizing the B lymphocyte lineage-specific tropism of KSHV. Here, we
S Ernst et al.
Critical reviews in biochemistry and molecular biology, 30(5), 387-444 (1995-01-01)
Glycosaminoglycans (GAGs) play an intricate role in the extracellular matrix (ECM), not only as soluble components and polyelectrolytes, but also by specific interactions with growth factors and other transient components of the ECM. Modifications of GAG chains, such as isomerization
Mi Gyeom Kim et al.
Scientific reports, 11(1), 3378-3378 (2021-02-11)
Cell surface heparan sulfate proteoglycan (HSPG)-mediated endocytosis results in poor yields of recombinant human bone morphogenetic proteins (rhBMPs) from CHO cell cultures. Upon incubation of rhBMP-2 and rhBMP-7 with CHO cells at 37 °C, both rhBMP-2 and rhBMP-7 bound to the
P M Galliher et al.
Applied and environmental microbiology, 41(2), 360-365 (1981-02-01)
Heparinase production by Flavobacterium heparinum in complex protein digest medium, with heparin employed as the inducer, has been studied and improved. The maximum productivity of heparinase has been increased 156-fold over that achieved by previously published methods to 375 U/liter
Yaron E Antebi et al.
Cell, 170(6), 1184-1196 (2017-09-09)
The bone morphogenetic protein (BMP) signaling pathway comprises multiple ligands and receptors that interact promiscuously with one another and typically appear in combinations. This feature is often explained in terms of redundancy and regulatory flexibility, but it has remained unclear
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Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
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