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Merck
CN

H3662

Sigma-Aldrich

HEPES 钠盐 溶液

1M, BioReagent, suitable for cell culture

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About This Item

经验公式(希尔记法):
C8H17N2NaO4S
CAS号:
分子量:
260.29
MDL编号:
UNSPSC代码:
12161700
PubChem化学物质编号:
NACRES:
NA.25

质量水平

无菌性

sterile-filtered (0.2 μm filtered)

产品线

BioReagent

形式

solution

浓度

0.90-1.10 M

技术

cell culture | mammalian: suitable

杂质

Bioburden, tested
DNase, RNase. Protease, Nickase, free
endotoxin, tested
<5 ppm Heavy metals (as Pb)

痕量阳离子

Fe: <5 ppm

SMILES字符串

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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一般描述

HEPES是一种不结合镁、钙、锰(II)或铜(II)离子的通用型两性离子缓冲剂。在1966年,Norman Good博士及其同事将HEPES归类为Good′s缓冲液。它在生物pH下为两性离子型,并在pH 6.8至8.2的范围内最有效。

应用

HEPES钠盐溶液适合用于细胞培养研究。它可用于制备HEPES缓冲液。它可在通透性成纤维细胞的磷酸化检测过程中用作缓冲液。它可被用于组成复杂的透析缓冲液,用于通过放射免疫检测对血清中的游离甲状腺素进行测定。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor
Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
S Hugel et al.
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar

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