质量水平
无菌性
sterile-filtered (0.2 μm filtered)
产品线
BioReagent
形式
solution
浓度
0.90-1.10 M
技术
cell culture | mammalian: suitable
杂质
Bioburden, tested
DNase, RNase. Protease, Nickase, free
endotoxin, tested
<5 ppm Heavy metals (as Pb)
痕量阳离子
Fe: <5 ppm
SMILES字符串
[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
InChI key
RDZTWEVXRGYCFV-UHFFFAOYSA-M
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一般描述
HEPES是一种不结合镁、钙、锰(II)或铜(II)离子的通用型两性离子缓冲剂。在1966年,Norman Good博士及其同事将HEPES归类为Good′s缓冲液。它在生物pH下为两性离子型,并在pH 6.8至8.2的范围内最有效。
应用
HEPES钠盐溶液适合用于细胞培养研究。它可用于制备HEPES缓冲液。它可在通透性成纤维细胞的磷酸化检测过程中用作缓冲液。它可被用于组成复杂的透析缓冲液,用于通过放射免疫检测对血清中的游离甲状腺素进行测定。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor
Hydrogen ion buffers for biological research.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar
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