H3159
Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
别名:
Anti-HD2, Anti-KDAC2, Anti-RPD3, Anti-YAF1
产品名称
Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 55 kDa
species reactivity
mouse, human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections
immunoprecipitation (IP): 50 μg using whole lysate of NIH3T3 cells
microarray: suitable
western blot: 1:20,000 using nuclear extract from human HeLa cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HDAC2(3066)
mouse ... Hdac2(15182)
Application
Anti-Histone Deacetylase 2 (HDAC2) antibody has been used:
- in immunoblotting and dot blot
- in immunoprecipitation
- in immunohistochemistry
- in enzyme linked immunosorbent assay (ELISA)
- in western blotting
- in chromatin immunoprecipitation(ChiP)
Biochem/physiol Actions
Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC2 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC2 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding. The antibody reacts with HDAC2 of human, rat and mouse origin.
Histone acetylation is a dynamic process whose levels are determined by the net activities of Histone acetyl transferase (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosome condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Histone Deacetylase (HDACs) have been described as six or seven different groups in mammals. HDAC1, HDAC2 and HDAC3 are like yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are like yeast Hda1 (Histone Deacetylase 1) protein.
Immunogen
synthetic peptide (C-SGEKTDTKGTKSEQLSNP) corresponding to the C-terminus region of histone deacetylase 2 of human origin (amino acids residues 471-488 with N-ternimal added cysteine) conjugated to maleimide-activated KLH. This sequence is highly conserved in mouse and chicken (2 amino acids and 1 amino acid substitutions, respectively).
Physical form
Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Histone deacetylase 2-mediated deacetylation of the glucocorticoid receptor enables NF-kappaB suppression
Ito K, et al.
The Journal of Experimental Medicine, 203(1), 7-13 (2006)
Anticancer activities of histone deacetylase inhibitors
Bolden J E, et al.
Nature Reviews Drug Discovery, 5(9), 769-769 (2006)
Development of an ELISA-based HDAC activity assay for characterization of isoform-selective inhibitors
Padige G, et al.
Journal of Biomolecular Screening, 20(10), 1277-1285 (2015)
The primary microRNA-208b interacts with Polycomb-group protein, Ezh2, to regulate gene expression in the heart
Mathiyalagan P, et al.
Nucleic Acids Research, 42(2), 790-803 (2013)
SAHA decreases HDAC 2 and 4 levels in vivo and improves molecular phenotypes in the R6/2 mouse model of Huntington's disease
Mielcarek M, et al.
PLoS ONE, 6(11), e27746-e27746 (2011)
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