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质量水平
方案
≥98% (HPLC)
表单
powder
反应适用性
reaction type: Biotinylations
颜色
white to off-white
mp
212 °C
溶解性
DMF: ≤50 mg/mL (Stable for at least one month in dry DMF)
DMSO: soluble
储存温度
−20°C
SMILES字符串
[H][C@]12CS[C@@H](CCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2
InChI
1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1
InChI key
YMXHPSHLTSZXKH-RVBZMBCESA-N
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应用
(+)-生物素NHS酯，或称(+)-生物素 N-羟基琥珀酰亚胺酯，已用于：
- 生物素化Hyb 246-4，用于表面活性剂蛋白 D 酶联免疫吸附试验（SP-D ELISA）
- 生物素化蛋白质以形成预成型络合物
- 标记和修饰单克隆抗微纤维相关蛋白 4 (MFAP4) (HG-HYB 7-18)，以结合到alphaLISA 链霉亲和素涂层供体珠
生化/生理作用
N-羟基琥珀酰亚胺酯（NHS）生物素修饰有助于目标赖氨酸残基在ε-氨基上的生物素化，并识别表面赖氨酸残基。它被用作识别蛋白质与几种生物分子相互作用的工具，如蛋白质-多糖相互作用、蛋白质-蛋白质相互作用、蛋白质-核酸相互作用和蛋白质-配体相互作用。NHS-生物素可用于蛋白和肽的生物素化。它通常在6.5-8.5的pH范围内通常偶联至伯胺。
免责声明
除非在我们的产品目录或产品随附的其他公司文档中另有说明，我们的产品仅供研究使用并不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途、任何类型的食用或应用于人或动物。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Helle Wulf-Johansson et al.
PloS one, 8(12), e82243-e82243 (2013-12-19)
Microfibrillar-associated protein 4 (MFAP4) is located in the extracellular matrix (ECM). We sought to identify tissues with high levels of MFAP4 mRNA and MFAP4 protein expression. Moreover, we aimed to evaluate the significance of MFAP4 as a marker of cardiovascular
M J Kim et al.
Analytical biochemistry, 231(2), 400-406 (1995-11-01)
Homogeneous-type enzyme-linked competitive binding assays utilizing the synthetic enzyme-biotin and avidin-riboflavin conjugates are developed for the detection of riboflavin as well as its binder protein. The activity of the enzyme-biotin conjugate is inhibited in the presence of the avidin-riboflavin conjugate
Hussein A Almehdar et al.
Probiotics and antimicrobial proteins, 12(1), 18-31 (2019-02-07)
Lactoferrin is an iron-binding glycoprotein present in various secretions (e.g., milk, tears, saliva, pancreatic juice), which performs multiple functions, with one of them being the antimicrobial defense. Purified camel lactoferrins (cLfs) from different Saudi camel clans, as well as human
Maria de Lourdes Muñoz et al.
BioMed research international, 2013, 875958-875958 (2013-12-11)
The main vector of dengue in America is the mosquito Aedes aegypti, which is infected by dengue virus (DENV) through receptors of midgut epithelial cells. The envelope protein (E) of dengue virus binds to receptors present on the host cells
Jes Sanddal Lindholt et al.
Journal of vascular surgery, 71(6), 1921-1929 (2019-12-01)
Identifying biomarkers for abdominal aortic aneurysms (AAA) could prove beneficial in prognosis of AAA and thus the selection for treatment. Microfibrillar-associated protein 4 (MFAP4) is an extracellular matrix protein that is highly expressed in aorta. MFAP4 is involved in several
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