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Merck
CN

H1506

3α-羟甾类脱氢酶 来源于睾丸酮假单胞菌

≥15 units/mg protein

别名:

3α-羟甾类:NAD(P)+氧化还原酶

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-839-5
MDL number:
Specific activity:
≥15 units/mg protein
Biological source:
bacterial (Pseudomonas testosteroni)
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biological source

bacterial (Pseudomonas testosteroni)

form

lyophilized solid

specific activity

≥15 units/mg protein

purified by

chromatography

composition

Protein, ≥25%

foreign activity

β-hydroxysteroid dehydrogenase ≤0.5%, alcohol dehydrogenase ≤0.05%

storage temp.

−20°C

Quality Level

Application

来源于假单胞菌 睾丸激素的3α-羟基类固醇脱氢酶已被用于:
  • 制备复合电极平台,以用于测定雄甾酮
  • 磷酸钾缓冲液中,以用于血清中胆汁酸的生物发光测定
  • 通过酶法测定总胆汁盐

Biochem/physiol Actions

3α-羟基类固醇脱氢酶 (3α-HSD) 可以帮助调节雄激素靶组织中雄激素受体 (AR) 的占据。它也可阻碍雄激素的作用。

Physical form

含有磷酸钾缓冲盐、EDTA和DL-二硫苏糖醇的冻干粉末

Other Notes

在β-NAD+存在,pH8.9,25℃条件下,一个单位每分钟可氧化1.0 μmole雄甾酮。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Chiaki Hidai et al.
Bioprocess and biosystems engineering, 32(5), 569-573 (2008-11-20)
Developing methods that result in targeting of therapeutic molecules in gene therapies to target tissues has importance, as targeting can increase efficacy and decrease off target-side-effects. Work from my laboratory previously showed that the extracellular matrix protein Del1 is organized
Xiang Li et al.
Archives of pharmacal research, 30(7), 812-815 (2007-08-21)
Two novel lactones, Cephalosporolides H (1) and 1 (2), were isolated from the lyophilized culture broth of the marine-derived fungus, Penicillium sp. The structures were elucidated on the basis of extensive 1D- and 2D-NMR, as well as HRESI-MS spectroscopic analyses.
Bin Li et al.
Proteins, 71(2), 670-683 (2007-11-03)
Experimentally determined protein tertiary structures are rapidly accumulating in a database, partly due to the structural genomics projects. Included are proteins of unknown function, whose function has not been investigated by experiments and was not able to be predicted by
Pascal Barat et al.
Endocrinology, 148(10), 4836-4843 (2007-07-14)
Glucocorticoids are metabolized by 11beta-hydroxysteroid dehydrogenase 1 (11betaHSD1) and the A-ring reductases (5alpha- and 5beta-reductases). Dysregulation of these enzymes has been reported in liver and adipose tissue in obese humans and animals, potentially leading to altered intracellular glucocorticoid concentrations and
Anne Z Steiner et al.
The Journal of clinical endocrinology and metabolism, 93(4), 1298-1303 (2008-02-07)
Dihydrotestosterone (DHT), the primary active androgen in peripheral target tissues, is metabolized by 3alpha-hydroxysteroid dehydrogenase type III (3alpha-HSD), encoded by the AKR1C2 gene, forming 5alpha-androstane-3alpha,17beta-diol (3alpha-diol). 3alpha-HSD may play a role in the pathogenesis of hirsutism. Our objective was to

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