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安全信息

H1029

Sigma-Aldrich

聚组氨酸单克隆抗体 小鼠抗

clone HIS-1, ascites fluid

别名:

聚组氨酸单克隆抗体, 6 His表位标签单克隆抗体, 6xHis标签单克隆抗体, HHHHHH表位标签单克隆抗体, Hexa His标签单克隆抗体, His6标签单克隆抗体, 组氨酸标签单克隆抗体, 聚组氨酸标签单克隆抗体

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.56

生物来源

mouse

质量水平

200

偶联物

unconjugated

抗体形式

ascites fluid

抗体产品类型

primary antibodies

克隆

HIS-1, monoclonal

形式

liquid

技术

dot blot: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

同位素/亚型

IgG2a

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

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一般描述

聚组氨酸（小鼠IgG2a）单克隆抗体可识别天然或变性的还原型合成聚组氨酸和聚组氨酸标签融合蛋白。 该抗体优先识别N-末端标签融合蛋白，并且与原核表达载体pET、pRSET和pTrc表达的融合蛋白反应。

特异性

该抗体可识别合成聚组氨酸，以及在指定载体中表达的天然或变性的还原型6X组氨酸标签蛋白。

免疫原

重组聚组氨酸标签融合蛋白。

应用

该抗体适用于免疫印迹、斑点杂交印迹、ELISA和免疫细胞化学分析。小鼠聚组氨酸单克隆抗体可用于蛋白质免疫印迹分析。

生化/生理作用

IgG2a（免疫球蛋白G 2a）基因主动参与刺激小鼠的自身免疫。该基因在病毒感染中高表达。

外形

以含有15mM叠氮化钠的腹水形式提供。

其他说明

用于免疫印迹的建议稀释度为1:3000

法律信息

本产品受专利DE 19507166以及独家授权给 QIAGEN GmbH（Qiagen Strasse 1, D-40724 Hilden, Germany）的国外同等专利的保护。

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WGK

WGK 1

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How many histidines are required for proper recognition by Product H1029, Monoclonal Anti-polyHistidine antibody?

    Product H1029, Monoclonal Anti-polyHistidine antibody requires at least 6 histidines in a row for recognition.

  6. What is the detection limit of Product H1029, Monoclonal Anti-polyHistidine antibody?

    The detection limit of product H1029 is 0.5 μg/ml or less.

  7. Is Product H1029, Monoclonal Anti-polyHistidine antibody, suitable for use in immunofluorescence studies?

    Product H1029 has been used in immunofluorescence and a good picture of the staining has been published. Ker R. Marshall, et al., Journal of General Virology 83, 1601-1612 (2002).

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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