form
suspension
technique(s)
affinity chromatography: suitable
matrix
Highly cross-linked 6% Beaded Agarose
capacity
15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
storage temp.
2-8°C
Quality Level
Application
亲和凝胶适用于在天然和变性条件下纯化组氨酸标记的蛋白质（His-tag蛋白）。
镍亲和凝胶用于纯化His-tag® 蛋白（组氨酸标记蛋白） 。 HIS-Select® 高流动性 (HF) 产品将HIS-Select 技术优良的选择性和高度交联的琼脂糖相结合，可在压力下实现更高的流速和机械稳定性。 HIS-Select HF产品设计用于生产规模的纯化和FPLC™ 应用。 和其他HIS-Select产品一样，次氮基三乙酸镍（Ni-NTA)）的螯合基团与琼脂糖的不带电亲水键可确保实现真正的一步纯化。
Features and Benefits
- 高选择性确保了高纯度
- 独特的不带电亲水键减少了非特异性结合
- 组氨酸标记蛋白（His-tag蛋白）的结合能力大于 15 mg/mL
- 高度交联的琼脂糖基质允许在更高压力下纯化
- 在变性或非变性条件下结合
- 一步纯化
General description
HIS-Select 高流动性镍亲和凝胶是一种固定化金属离子亲和色谱（IMAC）凝胶，设计用于特异性结合色谱系统中的His-tag蛋白（含组氨酸蛋白质），压力高达 200 psi，最大线性流速为 3,000 cm/hr。
Physical form
30% 乙醇溶液的混悬液 (1:1)
Preparation Note
使用前必须除去乙醇。用去离子水洗涤亲和凝胶以除去乙醇，然后用提供的平衡缓冲液进行平衡。
Legal Information
FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2
target_organs
Eyes,Central nervous system, Respiratory Tract
wgk
WGK 3
flash_point_f
88.0 °F
flash_point_c
31.1 °C
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
危险化学品
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利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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