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Merck
CN

GUSA

Sigma-Aldrich

β-Glucuronidase Fluorescent Activity Detection Kit

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UNSPSC代码:
12352200

用途

 kit sufficient for 200 standard tests

运输

dry ice

储存温度

−20°C

应用

For quantitative assay of β-glucuronidase gene products in plant or animal cell lysates.

特点和优势

  • Fluorescent detection for quantitative and qualitative measurement
  • Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
  • GUS does not interfere with plant cell function or viability

其他说明

The substrate used in this kit is MU-GlcA (4-methylumbelliferyl β-D-glucuronide), a widely used fluorogenic substrate for determination of glucuronidase activity.

仅试剂盒组分

产品编号
说明

  • Detailed technical bulletin 1 ea

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • 5× extraction buffer 25 mL

  • β-Glucuronidase 1000 U

  • M15084-MU 25 mg化学品安全说明书

  • 4-MU-GlcA 25 mg

  • Stop solution 100 mL

法规信息

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Current Protocols in Molecular Biology, 9-9 (1996)
An improved assay for beta-glucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous beta-glucuronidase activity. .
Kosugi, S., et al.
Plant Science, 70, 133-140 (1990)
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
Jefferson, R.A., et al.
The Embo Journal, 6, 390-3907 (1987)
R A Jefferson
Nature, 342(6251), 837-838 (1989-12-14)
The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.

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