usage
kit sufficient for 50 tests
Quality Level
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
UV/Vis spectroscopy: suitable
λmax
340 nm
application(s)
cell analysis
detection
storage temp.
2-8°C
Application
谷氨酰胺和谷氨酸测定试剂盒用于确定以下物质中L-谷氨酰胺的浓度：
- 最低必需培养基（MEM）培养基
- 上清液
- 血浆
该试剂盒通过将NAD还原为NADH，在339 nm波长处测定分光光度，用酶法测定L-谷氨酰胺和L-谷氨酸，
检测范围为：0.056-0.56 mM L-谷氨酸；0.14-1.4 mM L-谷氨酰胺。
检测范围为：0.056-0.56 mM L-谷氨酸；0.14-1.4 mM L-谷氨酰胺。
Biochem/physiol Actions
谷氨酰胺/谷氨酸测定试剂盒设计通过L-谷氨酰胺酶促脱氨、L-谷氨酸脱氢以及NAD+ 转为NADH后，用分光光度法测量L-谷氨酰胺和/或L-谷氨酸。该试剂盒可用于分光光度法测定液体制剂中的L-谷氨酰胺和/或L-谷氨酸。
signalword
Danger
存储类别
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
wgk
WGK 3
法规信息
常规特殊物品
危险化学品
此项目有
商品
Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.
本文介绍了增殖活性细胞为何需要碳源和氮源合成大分子。尽管大部分肿瘤细胞利用有氧糖酵解途径并分流线粒体氧化磷酸化代谢物，但许多肿瘤细胞表现出线粒体活性增加。
相关内容
Instructions
Wei Shi et al.
Oncology letters, 14(6), 6881-6887 (2017-11-23)
Glycolysis and glutaminolysis are heavily involved in the metabolic reprogramming of cancer cells. The activation of oncogenes and inactivation of tumor suppressor genes has a marked effect on the cellular metabolic processes glycolysis and glutaminolysis. N-Myc downstream-regulated gene 2 (NDRG2)
X Chang et al.
Clinical and experimental dermatology, 42(6), 614-621 (2017-06-10)
Malignant melanoma (MM) is an aggressive malignancy, which accounts for 80% of skin cancer-related deaths and is notably resistant to conventional chemotherapeutic agents. One of the most common treatments for melanoma is surgery, followed by various combinations of chemotherapy drugs.
Patricia Martins Bock et al.
Molecular and cellular biochemistry, 411(1-2), 351-362 (2015-11-05)
In this work, we aimed to investigate the effects of long-term supplementations with L-glutamine or L-alanyl-L-glutamine in the high-fat diet (HFD)-fed B6.129SF2/J mouse model over insulin sensitivity response and signaling, oxidative stress markers, metabolism and HSP70 expression. Mice were fed
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| GLN1-1KT | 04061833654828 |