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Merck
CN

GLN1

Sigma-Aldrich

谷氨酰胺和谷氨酸检测试剂盒

别名:

L-glutamine enzymatic quantification kit

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

用途

 kit sufficient for 50 tests

质量水平

包装

pkg of 1 kit

储存条件

dry at room temperature

技术

UV/Vis spectroscopy: suitable

λmax

340 nm

应用

cell analysis
detection

储存温度

2-8°C

应用

该试剂盒通过将NAD还原为NADH,在339 nm波长处测定分光光度,用酶法测定L-谷氨酰胺和L-谷氨酸,
检测范围为:0.056-0.56 mM L-谷氨酸;0.14-1.4 mM L-谷氨酰胺。
谷氨酰胺和谷氨酸测定试剂盒用于确定以下物质中L-谷氨酰胺的浓度:
  • 最低必需培养基(MEM)培养基
  • 上清液
  • 血浆

生化/生理作用

谷氨酰胺/谷氨酸测定试剂盒设计通过L-谷氨酰胺酶促脱氨、L-谷氨酸脱氢以及NAD+ 转为NADH后,用分光光度法测量L-谷氨酰胺和/或L-谷氨酸。该试剂盒可用于分光光度法测定液体制剂中的L-谷氨酰胺和/或L-谷氨酸。

警示用语:

Danger

危险分类

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品
危险化学品

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Gankyrin plays important roles in tumorigenicity and metastasis of hepatocellular carcinoma (HCC). We have for the first time investigated the effects of Gankyrin on glycolysis and glutaminolysis both in vitro and in vivo, including in patient-derived xenografts. We reported Gankyrin
Wei Shi et al.
Oncology letters, 14(6), 6881-6887 (2017-11-23)
Glycolysis and glutaminolysis are heavily involved in the metabolic reprogramming of cancer cells. The activation of oncogenes and inactivation of tumor suppressor genes has a marked effect on the cellular metabolic processes glycolysis and glutaminolysis. N-Myc downstream-regulated gene 2 (NDRG2)
L-Glutamine and L-glutamate: UV-method with glutaminase and glutamate dehydrogenase.
Lund, P. et al.
Methods of Enzymatic Analysis, 8, 357-363 (1986)
X Chang et al.
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Malignant melanoma (MM) is an aggressive malignancy, which accounts for 80% of skin cancer-related deaths and is notably resistant to conventional chemotherapeutic agents. One of the most common treatments for melanoma is surgery, followed by various combinations of chemotherapy drugs.

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