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保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pkg of 1 kit
制造商/商品名称
Cytiva RPN2235
相关类别
一般描述
Amersham™ ECL™ Select is a high sensitivity reagent for chemiluminescent Western blotting detection. High signal intensity makes Amersham™ ECL™ Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Its long lasting signals allow for multiple exposures and a convenient time window between experiment and analysis. Signals can be detected with CCD camera or X-ray film.
Amersham™ ECL™ Select typically exhibits a dynamic range that is complementary to Amersham™ ECL™ Prime, and similar to Amersham™ ECL™ Advance.
应用
特点和优势
- Improved sensitivity: Produces a very high signal intensity
- Quantitative measurements: Linear dynamic range allowing for quantitation of medium to very low abundant proteins
- Works with a wide range of antibody dilutions, reducing the need for lengthy optimization
- Optimized for imaging with ImageQuant LAS 4000 (CCD-based imaging) and compatible with Amersham™ Hyperfilm
- Compatible with Rainbow™ Molecular Weight Markers and Amersham™ ECL™ DualVue Western Blotting Markers.
- Optimized for use with Amersham™ Hybond-P (PVDF) membranes and compatible with Amersham™ Hybond® ECL™ (nitrocellulose) membranes
组分
Solution A (Luminol solution, 50 mL)
Solution B (Peroxide solution, 50 mL)
sufficient for 1000 cm2 membrane
complete packsize
for 1000 cm2 membrane
分析说明
法律信息
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
商品
描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
This page describes troubleshooting strategies in the detection of GST-tagged proteins.
实验方案
This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.
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