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安全信息

GERPN2106

ECL Western Blotting Reagents

Cytiva RPN2106

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UNSPSC代码:
41105332
NACRES:
NA.32

形式

liquid

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

特点

wetted part: no

包装

pkg of 1 kit

制造商/商品名称

Cytiva RPN2106

颜色

Detection reagent 1: Colorless Detection reagent 2: Colorless

溶解性

water: soluble

储存温度

2-8°C

相关类别

一般描述

Amersham ECL Western Blotting Detection Reagents were the world′s first commercial chemiluminescent reagents for Western blotting and are still the most widely used. They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time.

These reagents use enhanced luminol-based detection which is suitable for all routine western blotting. ECL Western Blotting Detection Reagents in combination with Hybond® ECL membranes form a complete solution for Western blot applications.

Emission duration max. 2 hours

Contents include the following:
GERPN2109, 62.5 mL each of ECL Detection Reagents 1 and 2 (1000 cm2)
GERPN2209, 125 mL each (2000 cm2)
GERPN2106, 250 mL each (4000 cm2)
GERPN2134, 375 mL each (6000 cm2).

应用

Western Blotting

特点和优势

  • High sensitivity, ~10 pg in model system.
  • Proven performance, the most frequently cited ECL Western Blotting Detection Reagents in publications
  • Detectable on Hyperfilm ECL or with CCD imager
  • Recommended membrane Hybond ECL

组分

Sufficient for detection of 4000 cm2 of membrane:
250 mL Detection reagent 1(color : colorless)
250 mL Detection reagent 2 (color: colorless)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

法规信息

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Sangphil Oh et al.
International journal of oncology, 57(6), 1319-1332 (2020-11-12)
ETS variant 1 (ETV1) is an oncogenic transcription factor. However, its role in colorectal cancer has remained understudied. The present study demonstrated that ETV1 downregulation led to reduced HCT116 colorectal cancer cell growth and clonogenic activity. Furthermore, the ETV1 mRNA
Linhua Wang et al.
Journal of cellular physiology, 236(6), 4625-4639 (2021-01-17)
Sepsis-induced myocardial dysfunction (SIMD), a deadly symptom in sepsis patients, is mainly caused by cardiovascular inflammation. However, it remains unclear how systemic inflammation triggers and aggravates cardiovascular inflammation in the pathogenesis of SIMD. This study found that proinflammatory cytokines and

商品

描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

实验方案

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

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