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GERPN1236

Anti-GST HRP Conjugate

Cytiva RPN1236, pack of 75 μL

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.46

偶联物

peroxidase conjugate

抗体产品类型

primary antibodies

包装

pack of 75 μL

制造商/商品名称

Cytiva RPN1236

颜色

Colorless

运输

dry ice

储存温度

−20°C

一般描述

Anti-GST HRP Conjugate.

应用

GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion Proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL Detection Reagents.
GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion Proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimized for use in Western blotting with ECL Detection Reagents.

特点和优势

  • Offers the advantages of speed, sensitivity, increased safety, and convenience when used with ECL detection systems. Application tested with ECL and ECL Plus reagents in western blotting for sensitive hard copy results
  • Detection of GST is simplified because no secondary antibody is required
  • The conjugate recognizes multiple epitopes of GST and therefore is not reliant on functional GST for fusion Protein detection. It has also been cross-absorbed to E. coli Proteins so that GST-fusion Proteins can be easily and specifically identified against an E. coli background.
  • Binds with high affinity to GST fusion Proteins.
  • Also suitable for colorimetric detection with diaminobenzidine (DAB) and tetramethylbenzidine (TMB).
  • ECL optimized protocols included.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

GST Gene Fusion Vectors
A license for commercial use of GST Gene Fusion Vectors under US patent 5,654,176 and equivalent patents and patent applications in other countries must be obtained from Millipore Corp (formerly Chemicon International Inc).
ECL is a trademark of Cytiva

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储存分类代码

12 - Non Combustible Liquids

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Asparagine endopeptidase cleaves α-synuclein and mediates pathologic activities in Parkinson's disease.
Zhang Z, et al.
Nature Structural and Molecular Biology, 24(8), 632-632 (2017)
Hannes Vanhaeren et al.
eLife, 9 (2020-03-27)
Protein ubiquitination is a very diverse post-translational modification leading to protein degradation or delocalization, or altering protein activity. In Arabidopsis thaliana, two E3 ligases, BIG BROTHER (BB) and DA2, activate the latent peptidases DA1, DAR1 and DAR2 by mono-ubiquitination at
Ibtissam Talhaoui et al.
Nature communications, 9(1), 5016-5016 (2018-11-28)
The repair of double-stranded DNA breaks (DSBs) by homologous recombination involves the formation of branched intermediates that can lead to crossovers following nucleolytic resolution. The nucleases Mus81-Mms4 and Yen1 are tightly controlled during the cell cycle to limit the extent
Zhentao Zhang et al.
Nature structural & molecular biology, 24(8), 632-642 (2017-07-04)
Aggregated forms of α-synuclein play a crucial role in the pathogenesis of synucleinopathies such as Parkinson's disease (PD). However, the molecular mechanisms underlying the pathogenic effects of α-synuclein are not completely understood. Here we show that asparagine endopeptidase (AEP) cleaves
Ying Chen et al.
Science advances, 10(11), eadj2570-eadj2570 (2024-03-13)
Ubiquitination plays a crucial role throughout plant growth and development. The E3 ligase DA2 has been reported to activate the peptidase DA1 by ubiquitination, hereby limiting cell proliferation. However, the molecular mechanisms that regulate DA2 remain elusive. Here, we demonstrate

商品

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

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