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生物来源
Escherichia coli
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 5000 units
制造商/商品名称
Cytiva E70073X
运输
dry ice
储存温度
−20°C
相关类别
一般描述
Acts specifically on single-stranded DNA degrading it processively in the 3′- to 5′-direction, producing 5′-mononucleotides.
Exonuclease I (Exo I) from E. coli is an Mg2+ ion dependent exonuclease.
应用
Exonuclease I has been used to quantify the expression of 56 genes in each F2 XY gonad.
生化/生理作用
Exonuclease I (Exo I) from E. coli can degrade single-stranded DNA (ssDNA) from 3′ to 5′. It participates in various DNA repair systems, like, RecBCD-dependent homologous recombination, methyl-dependent mismatch repair and genomic tandem repeat maintenance.
特点和优势
- Elimination of residual single-stranded DNA containing a 3′-terminus.
- Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA.
- Measuring DNA helicase activity.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法规信息
新产品
Structural and Biochemical Studies of a Moderately Thermophilic Exonuclease I from Methylocaldum szegediense
Fei L, et al.
Testing, 10(2), e0117470-e0117470 (2015)
Elucidation of the transcription network governing mammalian sex determination by exploiting strain-specific susceptibility to sex reversal
Munger S C, et al.
Genes & Development, 23(21), 2521-2536 (2009)
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