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GE29-0915-98

Superose^® 6 Increase

Name: Superose<SUP>®</SUP> 6 Increase
Brand: SIGMA

3.2/300, Cytiva 29-0915-98, 8.6 μm avg. part. size

别名:

Size exclusion chromatography, Superose 6 column

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About This Item

UNSPSC代码:

41115700

NACRES:

SB.52

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Sigma-Aldrich

GE29-0915-96

Superose^® 6 Increase 10/300 GL

Sigma-Aldrich

GE28-9909-44

Superdex^® 200 Increase 10/300 GL

Sigma-Aldrich

GE28-9909-46

Superdex^® 200 Increase 3.2/300

GE29323952

Superose^® 6 Prep Grade HiLoad^®

GE29321904

Superose^® 6 Increase HiScale^®

GE17-5172-01

Superose^® 6 10/300 GL

GE29321903

Superose^® 6 Increase HiScale^®

Sigma-Aldrich

GE28-9893-35

HiLoad^® 16/600 Superdex^® 200 pg

Sigma-Aldrich

GE28-9909-45

Superdex^® 200 Increase 5/150 GL

GE17-5175-01

Superdex^® 200 10/300 GL

物料

glass column

产品线

Superose^®

分子量

5000-5000000 Da (globular proteins)

制造商/商品名称

Cytiva 29-0915-98

参数

<0.03 mL/min flow rate (20% ethanol at low temp)
<0.07 mL/min flow rate (20% ethanol at room temp)
<0.07 mL/min flow rate (water at low temp)
<0.15 mL/min flow rate (water at room temp)
3 Mpa (435 psi)
4-50 μL sample volume

基质

cross-linked agarose
polymeric

基质活性基团

agarose phase

平均零件尺寸

8.6 μm

cleaning

1-14

工作范围

3-12

分离技术

size exclusion (SEC)

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一般描述

Superose^® 6 Increase 3.2/300 is a versatile, prepacked column for size exclusion chromatography in small-scale preparative purification (4 to 50 μl sample volume), as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. When high resolution is required, sample amount is limited and low buffer consumption is important, this is the column of choice.

特点和优势

Increased resolution compared with Superose 6, for higher purity and improved analysis results.
Reduced runtime compared with Superose 6, for rapid results.
Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

适用性

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

储存及稳定性

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Superdex is a registered trademark of Cytiva

Superose is a registered trademark of Cytiva

储存分类代码

3 - Flammable liquids

法规信息

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商品

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

实验方案

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

主要文件

安全信息

Superose^® 6 Increase