跳转至内容
Merck
CN
所有图片(2)

文件

安全信息

GE29-0491-04

HiTrap® MabSelect SuRe™

Cytiva 29-0491-04, pack of 1 mL

登录查看公司和协议定价
所有图片(2)

About This Item

UNSPSC代码:
23151816
NACRES:
NA.56

ligand

alkali-stabilized protein A-derived (E. coli)

包装

pack of 1 mL

制造商/商品名称

Cytiva 29-0491-04

存货情况

not available in North America

储存条件

(20% Ethanol)
 (20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)

参数

42 psi

柱床尺寸

7 mm × 25 mm

柱床体积

1 mL

色谱柱内径

7 mm

基质

highly cross-linked agarose

平均零件尺寸

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-13.7

working range

3-12

容量

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

适用性

suitable for bioprocess medium

储存温度

2-8°C

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allows cleaning-in-place (CIP).
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特点和优势

  • Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
  • Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Prepacked HiTrap columns for process development, screening of purification conditions, and small-scale purification of Mabs.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
HiTrap is a registered trademark of Cytiva
MabSelect SuRe is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

实验方案

Performing a Purification of IgG Antibodies with MabSelect Sure

This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from Cytiva.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门