所有图片(1)

主要文件

COO原产地证书/COA分析证书

安全信息

安全和法规

GE29-0485-86

HisTrap^™ Excel

Name: HisTrap<SUP>™</SUP> Excel
Brand: SIGMA

Cytiva 29-0485-86, pack of 1 mL

登录查看公司和协议定价

所有图片(1)

About This Item

UNSPSC代码:

23151816

NACRES:

NA.56

推荐产品

Slide 1 of 10

1 of 10

GE17-3712-06

HisTrap^™ Excel

GE29-0510-21

HisTrap^™ High Performance

GE17-5255-01

HisTrap^™ Fast Flow

GE17-5248-02

HisTrap^™ High Performance

GE17-5247-01

HisTrap^™ High Performance

GE17-5248-01

HisTrap^™高性能

GE17-3712-05

HisTrap^™ Excel

GE17-5319-01

HisTrap^™ Fast Flow

GE17-5468-55

HiTrap^® Capto^™ SP ImpRes

Roche

COHISC-RO

cOmplete^™His标签纯化色谱柱

包装

pack of 1 mL

制造商/商品名称

Cytiva 29-0485-86

存货情况

not available in North America

参数

1 ml/min - 4 ml/min flow rate
5 bar

柱床尺寸

7 mm × 25 mm

柱床体积

1 mL

色谱柱内径

7 mm

基质

highly cross-linked 6% agarose

平均直径

90 μm

cleaning

2-14

工作范围

2-13

一般描述

Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.

HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). HisTrap excel 1 mL and 5 mL are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications. The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins.

特点和优势

Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
Increase target protein yield and decrease degradation through reduced and simplified sample handling
HisTrap excel columns allow direct purification of cell-free, unclarified samples
HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

其他说明

H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.