推荐产品
ligand
recombinant protein G lacking albumin-binding region
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 1 mL
制造商/商品名称
Cytiva 29-0485-81
存货情况
not available in North America
储存条件
(20% Ehtanol)
(20% Ethanol)
参数
flow rate
42 psi
柱床尺寸
7 mm × 25 mm
柱床体积
1 mL
色谱柱内径
7 mm
基质
highly cross-linked 6% agarose
平均零件尺寸
34 μm
cleaning
2-9(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-9
容量
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
25 mg binding capacity(human IgG/column)
储存温度
2-8°C
相关类别
一般描述
特点和优势
34 μm bead size for high performance, high-resolution purifications.
Binds broad range of different species and subclasses of IgG.
Simple and proven method giving reproducible results.
Designed for use with syringe, peristalic pump, or chromatography system.
其他说明
法律信息
商品
Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
实验方案
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.
General instructions for affinity purification using HiTrap columns from Cytiva.
This page shows how to prepare samples for purification with affinity chromatography.
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