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GE29-0485-81

HiTrap® Protein G HP

Cytiva 29-0485-81, pack of 1 mL

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About This Item

UNSPSC代码:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

包装

pack of 1 mL

制造商/商品名称

Cytiva 29-0485-81

存货情况

not available in North America

储存条件

(20% Ehtanol)
 (20% Ethanol)

参数

flow rate
42 psi

柱床尺寸

7 mm × 25 mm

柱床体积

1 mL

色谱柱内径

7 mm

基质

highly cross-linked 6% agarose

平均零件尺寸

34 μm

cleaning

2-9

工作范围

3-9

容量

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
 25 mg binding capacity(human IgG/column)

储存温度

2-8°C

一般描述

HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特点和优势

High binding capacity of 25 mg IgG/ml medium and fast binding kinetics.
34 μm bead size for high performance, high-resolution purifications.
Binds broad range of different species and subclasses of IgG.
Simple and proven method giving reproducible results.
Designed for use with syringe, peristalic pump, or chromatography system.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

HiTrap is a registered trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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分析证书(COA)

Lot/Batch Number

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Philipp A Kronenberg et al.
Frontiers in cellular and infection microbiology, 13, 1162530-1162530 (2023-04-04)
Alveolar (AE) and cystic echinococcosis (CE) are severe parasitic zoonoses caused by the larval stages of Echinococcus multilocularis and E. granulosus sensu lato, respectively. A panel of 7 monoclonal antibodies (mAbs) was selected against major diagnostic epitopes of both species.

商品

Automated Antibody Purification with ÄKTA Systems

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

Performing a Separation with HiTrap® Protein G HP

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

查看所有结果

实验方案

Two-step Purification of Mouse Monoclonal IgG1 Using HiTrap® Protein G HP for the Capture Step

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

General Instructions for Affinity Purification Using HiTrap® Columns

General instructions for affinity purification using HiTrap columns from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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