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GE29-0485-65

HiTrap® TALON® Crude

Cytiva 29-0485-65, pack of 1 mL

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UNSPSC代码:
23151816
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包装

pack of 1 mL

制造商/商品名称

Cytiva 29-0485-65

存货情况

not available in North America

参数

1 ml/min - 4 ml/min flow rate
3 bar

色谱柱内径

7 mm

基质

6% cross-linked agarose

粒径

60-160 μm

cleaning

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

容量

≥20 mg binding capacity (histidine-tagged protein)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)

储存温度

2-8°C

相关类别

一般描述

HiTrap TALON crude 1 ml and 5 ml columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment. TALON Superflow™ is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. TALON Superflow and prepacked HiTrap TALON crude columns allow protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

应用

HiTrap TALON crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the TALON Superflow beads. Prior to loading samples on HiTrap TALON crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.

特点和优势

  • Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.
  • Prepacked HiTrap TALON crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.
  • HiTrap TALON crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

HiTrap is a registered trademark of Cytiva
Superflow is a trademark of Sterogene Bioseparations Inc.
Talon is a registered trademark of Clontech

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

法规信息

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商品

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

实验方案

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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