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GE29-0485-65

HiTrap^® TALON^® Crude

Name: HiTrap<SUP>®</SUP> TALON<SUP>®</SUP> Crude
Brand: SIGMA

Cytiva 29-0485-65, pack of 1 mL

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About This Item

UNSPSC代码:

23151816

NACRES:

NA.56

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TALON^® Superflow Cobalt-IMAC

包装

pack of 1 mL

制造商/商品名称

Cytiva 29-0485-65

存货情况

not available in North America

参数

1 ml/min - 4 ml/min flow rate
3 bar

色谱柱内径

7 mm

基质

6% cross-linked agarose

粒径

60-160 μm

cleaning

2-14(Co²⁺-stripped medium)

工作范围

3-12(Co2+-stripped medium)

容量

≥20 mg binding capacity (histidine-tagged protein)

储存温度

2-8°C

一般描述

HiTrap TALON crude 1 ml and 5 ml columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment. TALON Superflow^™ is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. TALON Superflow and prepacked HiTrap TALON crude columns allow protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

应用

HiTrap TALON crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the TALON Superflow beads. Prior to loading samples on HiTrap TALON crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.

特点和优势

Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).
Suitable for IMAC purification of histidine-tagged proteins when Ni²⁺ is not the optimal choice of metal ion.
Compatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.
Prepacked HiTrap TALON crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.
HiTrap TALON crude columns allow repeated runs without stripping, cleaning, or Co²⁺ recharging.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.