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安全信息

GE29-0005-93

His Spintrap™ TALON®

Cytiva 29-0005-93

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UNSPSC代码:
41106500
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

保质期

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

制造商/商品名称

Cytiva 29-0005-93

包装

pkg of 50 x 100 μL

柱床体积

100 μL

基质

6% cross-linked agarose

粒径

60-160 μm

cleaning

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

容量

≤1 mg binding capacity(histidine-tagged)
 ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

储存温度

2-8°C

相关类别

一般描述

His SpinTrap™ Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.

应用

His SpinTrap™ Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap™ Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.

特点和优势

  • Suitable for purification of multiple samples in parallel, such as screening.
  • Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
  • With a microcentrifuge, one run takes about 10 minutes.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

SpinTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

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商品

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

实验方案

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

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