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ligand
butyl
包装
pack of 1 column
制造商/商品名称
Cytiva 28-9924-73
柱床尺寸
7.7 mm × 100 mm
色谱柱内径
7.7 mm
基质
highly cross-linked agarose
平均零件尺寸
75 μm (d50v)
cleaning
3-14
工作范围
3-13
容量
27 mg binding capacity(27 mg BSA /ml (at QB10%))
适用性
suitable for bioprocess medium
一般描述
Capto™ Phenyl (High sub) and Capto™ Butyl are hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification.
应用
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
Capto™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose™ 4 Fast Flow. Higher flow rates can be used with Capto™ Butyl during equilibration, wash and regeneration.
Capto™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose™ 4 Fast Flow. Higher flow rates can be used with Capto™ Butyl during equilibration, wash and regeneration.
特点和优势
- Prepacked with Capto™ Butyl
- Excellent for method optimization and parameter screening due to the 10 cm bed height
- Easily connected in series to achieve 20 cm bed height
- Small bed volume for fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
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