packaging
pack of 1 kit
manufacturer/tradename
Cytiva 28-9805-82
parameter
Ambient temp. range
matrix
highly cross-linked spherical agarose (Sepharose) including magnetite
capacity
~50 mg binding capacity(histidine-tagged protein/ml medium (~500 μg/purification run))
storage temp.
2-8°C
General description
Membrane protein Purification Kit for simultaneously detergent screening and purification of histidine-tagged membrane proteins.
Application
Membrane Protein Purification Kit is designed for efficient detergent screening of histidine-tagged membrane Proteins. Small aliquots of cell membranes are solubilized in different detergents followed by rapid purification using His Mag Sepharose™ Ni. The purification step is performed directly after solubilization using the same detergent. Analysis and evaluation can be performed by a number of methods such as Western blot, gel filtration, or light scattering. Membrane Protein Purification Kit is sufficient for two complete screening experiments according to the included protocol.
His Mag Sepharose™ Ni, is a magnetic bead format excellent for small-scale experiments. The high density of the beads allows rapid capture by magnetic devices, while the visibility of the beads ensures reliable collection of the bound histidine-tagged membrane Proteins in the screening and purification procedure. Purification of target membrane Proteins for a number of analytical and/or other downstream applications can be achieved within a few hours. For convenient purification of larger amounts of membrane Proteins, use prepacked HisTrap™ HP columns.
His Mag Sepharose™ Ni, is a magnetic bead format excellent for small-scale experiments. The high density of the beads allows rapid capture by magnetic devices, while the visibility of the beads ensures reliable collection of the bound histidine-tagged membrane Proteins in the screening and purification procedure. Purification of target membrane Proteins for a number of analytical and/or other downstream applications can be achieved within a few hours. For convenient purification of larger amounts of membrane Proteins, use prepacked HisTrap™ HP columns.
Features and Benefits
- Simplify detergent screening of histidine-tagged membrane Proteins.
- Simultaneous evaluation of solubilization and purification efficiency.
- Rapid and easy screening in the convenient magnetic bead format.
- Ready-to-use detergent solutions.
- Easy-to-folLow protocol.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
存储类别
11 - Combustible Solids
法规信息
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