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安全信息

GE28-9782-45

HiScreen™, DEAE Fast Flow

Cytiva 28-9782-45

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About This Item

UNSPSC代码:
47101511
NACRES:
NA.56

ligand

diethylaminoethyl

描述

Ion Exchanger Type (value)

表单

prepacked column

交联

cross-linked

包装

pkg of 1 ea

制造商/商品名称

Cytiva 28-9782-45

储存条件

(20% Ethanol)

参数

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

技术

liquid chromatography (LC): suitable

柱床尺寸

7.7 mm × 100 mm

柱床体积

4.7 mL

色谱柱内径

7.7 mm

基质

6% cross-linked agarose

基质活性基团

N+(C2H5)2H functional group

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14

工作范围

2-12

容量

binding capacity(~ 110 mg HAS/mL resin)

适用性

suitable for bioprocess medium

分离技术

anion exchange

相关类别

一般描述

HiScreen™ DEAE FF columns are prepacked with DEAE Sepharose Fast Flow, a weak anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

应用

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

特点和优势

  • DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
  • Prepacked, ready-to-use columns for convenient process development optimization.
  • 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
  • Easily connected in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption.
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

实验方案

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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