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GE28-9782-44

HiScreen™ Ni Fast Flow

Cytiva 28-9782-44

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About This Item

UNSPSC代码:
13111000
NACRES:
NA.56

包装

pkg of 1 ea

制造商/商品名称

Cytiva 28-9782-44

参数

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

柱床尺寸

7.7 mm × 100 mm

柱床体积

4.7 mL

色谱柱内径

7.7 mm

基质

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14(Ni2+-stripped medium.)

工作范围

3-12(Ni2+-stripped medium.)

容量

~40 mg binding capacity(histidine-tagged protein)

适用性

suitable for bioprocess medium

相关类别

一般描述

HiScreen™ columns prepacked with Ni Sepharose™ 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

应用

HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen™ Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose™ 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.

特点和优势

  • High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
  • Prepacked, ready-to-use columns for convenient process development.
  • Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
  • Easy connection in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形图

Environment
GHS09

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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历史批次信息供参考:

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Lot/Batch Number

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商品

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

实验方案

Ni Sepharose 6 Fast Flow for Protein Purification

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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