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Cibacron Blue
包装
pkg of 1 ea
制造商/商品名称
Cytiva 28-9782-43
储存条件
(20% Ethanol, 0.1 M potassium phosphate)
(20% Ethanol, 0.9% NaCl, 1 mM Ca2₊ and 1 mM Mn2₊)
参数
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
柱床尺寸
7.7 mm × 100 mm
柱床体积
4.7 mL
色谱柱内径
7.7 mm
基质
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
3-13
工作范围
4-12
适用性
suitable for bioprocess medium
储存温度
2-8°C
一般描述
HiScreen™ columns prepacked with Blue Sepharose™ Fast FLow are an excellent choice for method optimization and parameter screening for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons, and related proteins.
应用
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
HiScreen™ Blue FF is prepacked with the BioProcess chromatography medium Blue Sepharose™ 6 Fast Flow. This medium has Cibacron Blue 3G covalently attached to the Sepharose™ 6 Fast FLow matrix by the triazine coupling method. The Blue dye binds many Proteins, such as albumin, interferon, lipoProteins, and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors (e.g., NAD).
HiScreen™ Blue FF is prepacked with the BioProcess chromatography medium Blue Sepharose™ 6 Fast Flow. This medium has Cibacron Blue 3G covalently attached to the Sepharose™ 6 Fast FLow matrix by the triazine coupling method. The Blue dye binds many Proteins, such as albumin, interferon, lipoProteins, and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors (e.g., NAD).
特点和优势
- Can be used for depletion of albumin.
- Prepacked, ready-to-use columns for convenient process development.
- Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
- Easy connection in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
新产品
商品
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
实验方案
This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.
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