推荐产品
ligand
protein G
包装
pack of 2 × 1 mL
制造商/商品名称
Cytiva 28-9670-66
储存条件
(20% Ethanol, 10% medium slurry)
参数
Room temperature temp. range
基质
paramagnetic, spherical, highly cross-linked agarose particles
容量
27 mg binding capacity(human IgG/ml gel)
储存温度
2-8°C
一般描述
应用
Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose™ Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose™ medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.
特点和优势
- Efficient, high capacity small-scale purification and screening of antibodies from various species; 27 μg human IgG/μL magnetic beads
- High purity and yield
- Robust parallel screening of antibodies with high reproducibility
- Simple capture of antibodies from small or large sample volumes (low-microliter to high-milliliter scale)
- Visible and dense Sepharose™ based magnetic beads give increased simplicity for handling
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.
储存及稳定性
分析说明
法律信息
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
实验室规模的脱盐是一种成熟、简单、快速的方法,可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
实验方案
Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.
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