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安全信息

GE28-9644-41

Sigma-Aldrich

HiScale 16/20

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UNSPSC代码:
12352200
NACRES:
SB.52

技术

LPLC: suitable

一般描述

HiScale columns are delivered with a column tube, outer protection tube, two adapters, PEEK tubing, and unions at both ends for easy connection to valves, pumps, and monitors.
HiScale columns are designed to withstand pressures of up to 20 bar (290 psi) and high flow rates, making them compatible with modern BioProcess chromatography media such as MabSelect and Capto. The user-friendly design allows for an optimized process, through simplified and reproducible operation.

特点和优势

Adapter QuickLock mechanism, column measurement scale, and ergonomic design, facilitate column handling and increase process control.
Based on the well-established line of XK columns and compatible with all methods currently applicable to XK columns.
Capability to rinse entire adapter shaft after packing, thereby minimizing risk of bacterial growth after prolonged use.
Dual adapters, offering high flexibility and dynamic packing heights.
Empty columns with high pressure stability for packing with modern BioProcess media for process development and preparative chromatography.
Provides axial compression of the gel bed and a nonrotating plunger mechanism that expands packing capabilities.

法规信息

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This page describes efficient column packing and preparation for affinity chromatography of antibodies.

本页介绍了高效的色谱柱填充和抗体亲和层析的制备。

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

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实验方案

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

This page describes efficient column packing and preparation for affinity chromatography of GST-tagged proteins using Cytiva products.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

Column Packing and Preparation for Size Exclusion Chromatography

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