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TALON® Superflow Cobalt-IMAC
保质期
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 50 mL
制造商/商品名称
Cytiva 28-9575-02
基质
6% cross-linked agarose
粒径
60-160 μm
cleaning in place
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
容量
≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
储存温度
2-8°C
相关类别
一般描述
特点和优势
- Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
- Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
分析说明
法律信息
商品
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
实验方案
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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