ligand
TALON® Superflow Cobalt-IMAC
包装
pack of 10 mL
制造商/商品名称
Cytiva 28-9574-99
基质
6% cross-linked agarose
粒径
60-160 μm
cleaning in place
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
容量
≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
储存温度
2-8°C
相关类别
一般描述
特点和优势
- Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
- Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
储存及稳定性
分析说明
法律信息
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
实验方案
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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