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安全信息

GE28-9523-59

GST Spintrap™

Cytiva 28-9523-59

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

ligand

glutathione

包装

pkg of 50 ea

制造商/商品名称

Cytiva 28-9523-59

柱床体积

50 μL

基质

4% agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

4-13

工作范围

4-13

容量

>25 mg binding capacity (horse liver GST/ml medium)
 ≥500 μg binding capacity(recombinant glutathione S-transferase (rGST))

一般描述

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST SpinTrap columns are designed for rapid small-scale purification of GST-tagged proteins directly from clarified or unclarified cell lysates. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves the functionality of the proteins, and more than 90% purity can be obtained in a single step. GST SpinTrap columns are suitable for purification of multiple samples in parallel, for example in screening experiments such as evaluation of chromatography media, growth temperature, culture density, and other variables to find optimal conditions for expression.

特点和优势

  • Preserves protein antigenicity and function.
  • Yields up to 500 μg of pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (PBS and 0,05% Kathon CG/ICP Biocide)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

SpinTrap is a trademark of Cytiva

储存分类代码

12 - Non Combustible Liquids

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Purification using GST SpinTrap™

This page shows how to purify GST-tagged proteins using GST SpinTrap™ from Cytiva.

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

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实验方案

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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